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Literature summary extracted from

  • Greenberg, R.; Dudock, B.
    Bacterial tRNA methyltransferases (1979), Methods Enzymol., 59, 190-203.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.35 molecular biology enzyme is used for detecting tRNA-like moieties in viral RNA Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.35 S-adenosyl-L-homocysteine at 0.002 mM 50% inhibition, E. coli MRE 600 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.35 soluble
-
 Escherichia coli
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.35 Bacillus cereus
-
-
-
2.1.1.35 Bacillus subtilis
-
-
-
2.1.1.35 Enterococcus faecalis
-
-
-
2.1.1.35 Escherichia coli
-
 MRE 600
-
2.1.1.35 Escherichia coli MRE 600
-
 MRE 600
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.35 affinity elution and hydroxylapatite chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.35 0.00134
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.1.35 -70°C, at least 6 months, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.35 additional information
-
 Escherichia coli additional information
-
 ?
2.1.1.35 additional information tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine Bacillus subtilis additional information
-
 ?
2.1.1.35 additional information tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine Enterococcus faecalis additional information
-
 ?
2.1.1.35 additional information tetrahydrofolate derivatives as methyl group donor in the formation of 5-methyluridine Bacillus cereus additional information
-
 ?
2.1.1.35 additional information
-
 Escherichia coli MRE 600 additional information
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA high substrate specificity, methyl group acceptors: wheat germ tRNA1Gly, tRNA2Gly, E. coli tRNA1Ala Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA high substrate specificity, methyl group acceptors: wheat germ tRNA1Gly, tRNA2Gly, E. coli tRNA1Ala Escherichia coli MRE 600 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.35 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.35 8.4
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.35 S-adenosyl-L-methionine
-
 Escherichia coli