BRENDA - Enzyme Database

Plant methionine synthase: new insights into properties and expression

Eckermann, C.; Eichel, J.; Schroder, J.; Biol. Chem. 381, 695-703 (2000)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.08
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
Catharanthus roseus
2.1.1.14
0.35
-
5-methyltetrahydropteroylmonoglutamate
-
Catharanthus roseus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.14
Mg2+
no stimulation
Catharanthus roseus
2.1.1.14
phosphate
-
Catharanthus roseus
2.1.1.14
Zn2+
-
Catharanthus roseus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.14
85000
-
gel filtration, recombinant enzyme
Catharanthus roseus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.14
Catharanthus roseus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.14
cell culture
-
Catharanthus roseus
-
2.1.1.14
cotyledon
-
Catharanthus roseus
-
2.1.1.14
hypocotyl
-
Catharanthus roseus
-
2.1.1.14
seedling
low activity
Catharanthus roseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441337
Catharanthus roseus
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
441337
Catharanthus roseus
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.14
monomer
1 * 85000, SDS-PAGE, recombinant enzyme
Catharanthus roseus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.08
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
Catharanthus roseus
2.1.1.14
0.35
-
5-methyltetrahydropteroylmonoglutamate
-
Catharanthus roseus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.14
Mg2+
no stimulation
Catharanthus roseus
2.1.1.14
phosphate
-
Catharanthus roseus
2.1.1.14
Zn2+
-
Catharanthus roseus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.14
85000
-
gel filtration, recombinant enzyme
Catharanthus roseus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.14
cell culture
-
Catharanthus roseus
-
2.1.1.14
cotyledon
-
Catharanthus roseus
-
2.1.1.14
hypocotyl
-
Catharanthus roseus
-
2.1.1.14
seedling
low activity
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441337
Catharanthus roseus
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
441337
Catharanthus roseus
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.14
monomer
1 * 85000, SDS-PAGE, recombinant enzyme
Catharanthus roseus