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Human liver nicotinamide N-methyltransferase. cDNA cloning, expression, and biochemical characterization

Aksoy, S.; Szumlanski, C.L.; Weinshilboum, R.M.; J. Biol. Chem. 269, 14835-14840 (1994)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.1
-
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.1
N1-methylnicotinamide
IC50 0.06 mM for human protein, 0.03 mM for recombinant protein
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.1
0.0018
-
S-adenosyl-L-methionine
-
Homo sapiens
2.1.1.1
0.0022
-
S-adenosyl-L-methionine
recombinant protein
Homo sapiens
2.1.1.1
0.38
-
nicotinamide
recombinant protein
Homo sapiens
2.1.1.1
0.43
-
nicotinamide
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.1
29000
-
x * 29000, SDS-PAGE, recombinant protein
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.1
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
441255
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.1
?
x * 29000, SDS-PAGE, recombinant protein
Homo sapiens
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.1.1.1
0.06
-
IC50 0.06 mM for human protein, 0.03 mM for recombinant protein
Homo sapiens
N1-methylnicotinamide
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.1
-
Homo sapiens
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.1.1.1
0.06
-
IC50 0.06 mM for human protein, 0.03 mM for recombinant protein
Homo sapiens
N1-methylnicotinamide
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.1
N1-methylnicotinamide
IC50 0.06 mM for human protein, 0.03 mM for recombinant protein
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.1
0.0018
-
S-adenosyl-L-methionine
-
Homo sapiens
2.1.1.1
0.0022
-
S-adenosyl-L-methionine
recombinant protein
Homo sapiens
2.1.1.1
0.38
-
nicotinamide
recombinant protein
Homo sapiens
2.1.1.1
0.43
-
nicotinamide
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.1
29000
-
x * 29000, SDS-PAGE, recombinant protein
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
441255
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.1
?
x * 29000, SDS-PAGE, recombinant protein
Homo sapiens