BRENDA - Enzyme Database

Isolation of a cobalamin containing 5-methyltetrahydrofolate-homocysteine transmethylase from mammalian kidney

Mangum, J.H.; North, J.A.; Biochemistry 10, 3765-3769 (1971)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.1.1.13
homocysteine, 0.01 M, stabilizes
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.13
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.13
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.13
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.13
additional information
-
-
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
2.1.1.13
-20°C, ammonium sulfate paste containing homocysteine
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.13
N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
441180
Sus scrofa
tetrahydropteroyltriglutamate + L-methionine
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.13
additional information
absolute requirement for a reducing system
Sus scrofa
2.1.1.13
S-adenosyl-L-methionine
partially stimulates
Sus scrofa
2.1.1.13
vitamin B12
enzyme contains a derivative of vitamin B12 as prosthetic group
Sus scrofa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.13
additional information
absolute requirement for a reducing system
Sus scrofa
2.1.1.13
S-adenosyl-L-methionine
partially stimulates
Sus scrofa
2.1.1.13
vitamin B12
enzyme contains a derivative of vitamin B12 as prosthetic group
Sus scrofa
General Stability (protein specific)
EC Number
General Stability
Organism
2.1.1.13
homocysteine, 0.01 M, stabilizes
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.13
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.13
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.13
additional information
-
-
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.1.1.13
-20°C, ammonium sulfate paste containing homocysteine
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.13
N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
441180
Sus scrofa
tetrahydropteroyltriglutamate + L-methionine
-
-
-
?