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Literature summary extracted from

  • Sztajer, H.; Wang, W.; Luensdorf, H.; Stocker, A.; Schmid, R.D.
    Purification and some properties of a novel microbial lactate oxidase (1996), Appl. Microbiol. Biotechnol., 45, 600-606.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.15 addition of 0.03 mM FMN increases the stability about 30% after 10 d of storage Geotrichum candidum
1.1.3.15 addition of MnCl2, Dextran 500000, glycerol or octyl glucopyranoside increase stability after 10 d of incubation at 4°C, HgCl2 and SDS destabilizes Geotrichum candidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 3.6
-
 (S)-lactate
-
 Geotrichum candidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.15 50000
-
 8 * 50000, transmission electron microscopy analysis and SDS-PAGE Geotrichum candidum
1.1.3.15 400000
-
 native PAGE Geotrichum candidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.15 (S)-lactate + O2 Geotrichum candidum
-
 pyruvate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Geotrichum candidum
-
 strain DSM 10452
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15
-
 Geotrichum candidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.15 60.7
-
-
 Geotrichum candidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 (S)-lactate + O2
-
 Geotrichum candidum pyruvate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.15 octamer 8 * 50000, transmission electron microscopy analysis and SDS-PAGE Geotrichum candidum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.15 8 11 stable for 15 min at 30°C Geotrichum candidum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN 0.125 mol 5'-FMN per monomer, not covalently bound Geotrichum candidum