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Literature summary extracted from

  • Yorita, K.; Janko, K.; Aki, K.; Ghisla, S.; Palfey, B.A.; Massey, V.
    On the reaction mechanism of L-lactate oxidase: quantitative structure-activity analysis of the reaction with para-substituted L-mandelates (1997), Proc. Natl. Acad. Sci. USA, 94, 9590-9595.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.15 A95G enhanced activity with bulky substrates, increase in the reduction rate constants with substrates having a bulky alpha-substituent such as mandelate, when compared with wild-type enzyme Aerococcus viridans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 additional information
-
 additional information reduction rate constants and dissociation constants for reaction of lactate oxidases with alpha-1H- and alpha-2H-para-substituted mandelates Aerococcus viridans

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Aerococcus viridans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 DL-mandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans phenylglyoxylic acid + H2O2
-
 ?
1.1.3.15 DL-p-hydroxymandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
1.1.3.15 L-4-chloromandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
1.1.3.15 L-4-fluoromandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
1.1.3.15 L-4-methoxymandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
1.1.3.15 L-4-methylmandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
1.1.3.15 L-4-nitromandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
1.1.3.15 L-4-trifluoromethylmandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
1.1.3.15 L-mandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.15 25
-
 assay at Aerococcus viridans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.15 additional information
-
 additional information reduction rate constants and dissociation constants for reaction of lactate oxidases with alpha-1H- and alpha-2H-para-substituted mandelates Aerococcus viridans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.15 7
-
 assay at Aerococcus viridans