EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.21.3.1 | ascorbic acid | 5fold stimulation | Flavobacterium sp. | |
1.21.3.1 | dithioerythritol | absolutely required, highly stimulating | Flavobacterium sp. | |
1.21.3.1 | O2 | required | Flavobacterium sp. | |
1.21.3.1 | polyethylene glycol 1500 | 20% stimulation up to concentration of 5% | Flavobacterium sp. | |
1.21.3.1 | Triton X-100 | leads to 50% stimulation at concentration of 0.01% | Flavobacterium sp. |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.21.3.1 | pharmacology | model system for study of endogenous functions of beta-lactams in bacteria | Flavobacterium sp. |
EC Number | General Stability | Organism |
---|---|---|
1.21.3.1 | unstable to 20 mM dithiothreitol during storage at -20°C, 10% activity remaining | Flavobacterium sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.21.3.1 | Co2+ | moderate inhibition | Flavobacterium sp. | |
1.21.3.1 | cystamine | 100% inhibition at 0.5 mM | Flavobacterium sp. | |
1.21.3.1 | cysteamine | 43% inhibition at 1.5 mM | Flavobacterium sp. | |
1.21.3.1 | cysteine | 60% inhibition at 1 mM | Flavobacterium sp. | |
1.21.3.1 | cystine | 81% inhibition at 1.5 mM | Flavobacterium sp. | |
1.21.3.1 | GSH | 70% inhibition at 1 mM | Flavobacterium sp. | |
1.21.3.1 | Mn2+ | moderate inhibition | Flavobacterium sp. | |
1.21.3.1 | additional information | ATP, NADPH and FAD: no effect; cations Na+, K+, Mg2+, Ca2+, Fe3+ do not affect the activity | Flavobacterium sp. | |
1.21.3.1 | Ni2+ | moderate inhibition | Flavobacterium sp. | |
1.21.3.1 | Triton X-100 | inhibits at concentration of 0.5% | Flavobacterium sp. | |
1.21.3.1 | Zn2+ | moderate inhibition | Flavobacterium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.21.3.1 | 0.08 | - |
delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine | - |
Flavobacterium sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.21.3.1 | cytosol | - |
Flavobacterium sp. | 5829 | - |
1.21.3.1 | membrane | inactive associated form | Flavobacterium sp. | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.21.3.1 | Fe2+ | stimulates | Flavobacterium sp. | |
1.21.3.1 | Fe2+ | low concentration | Flavobacterium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.21.3.1 | 36500 | 38000 | gel filtration and SDS-PAGE | Flavobacterium sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Flavobacterium sp. | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Flavobacterium sp. SC 12.154 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | product has antibiotic activity | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.21.3.1 | Flavobacterium sp. | - |
- |
- |
1.21.3.1 | Flavobacterium sp. SC 12.154 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.21.3.1 | - |
Flavobacterium sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.21.3.1 | 0.00129 | - |
about, purified enzyme | Flavobacterium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.3.1 | delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine + O2 | - |
Flavobacterium sp. | isopenicillin N + H2O | - |
r | |
1.21.3.1 | delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine + O2 | - |
Flavobacterium sp. SC 12.154 | isopenicillin N + H2O | - |
r | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Flavobacterium sp. | isopenicillin N + 2 H2O | - |
? | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Flavobacterium sp. | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Flavobacterium sp. SC 12.154 | isopenicillin N + 2 H2O | - |
? | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Flavobacterium sp. SC 12.154 | isopenicillin N + 2 H2O | product has antibiotic activity | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.21.3.1 | monomer | 1 * 36500-38000, SDS-PAGE and gel filtration | Flavobacterium sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.21.3.1 | IPNS | - |
Flavobacterium sp. |
1.21.3.1 | isopenicillin N-synthase | - |
Flavobacterium sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.21.3.1 | 15 | 20 | - |
Flavobacterium sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.21.3.1 | 7 | 7.5 | - |
Flavobacterium sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.21.3.1 | 6 | 9 | 30% activity remaining above pH 9.0, highly reduced activity below pH 6.0 | Flavobacterium sp. |