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Literature summary extracted from

  • Gerber, N.C.; Nishida, C.R.; de Montellano, P.R.O.
    Characterization of human liver inducible nitric oxide synthase expressed in Escherichia coli (1997), Arch. Biochem. Biophys., 343, 249-253.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 inducible liver isoform is expressed in Escherichia coli with and without His-tag, requires coexpression of calmodulin Homo sapiens

General Stability

EC Number General Stability Organism
1.14.13.39 inducible isoform is unstable during purification in absence of L-arginine and tetrahydrobiopterin toward loss of the heme group and formation of low-spin species Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 0.022
-
 L-arginine in presence of tetrahydrobiopterin and L-arginine Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ no stimulation with exogenous Ca2+, inducible isoform from liver Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Homo sapiens
-
 inducible
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 recombinant wild-type of inducible liver isoform from Escherichia coli with and without His-tag, requires inclusion of tetrahydrobiopterin in purification buffer Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 liver inducible isoform Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 38
-
 purified enzyme, inducible isoform, cytochrome c reductase activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Homo sapiens 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 Calmodulin no stimulation with exogenous calmodulin, inducible isoform from liver Homo sapiens
1.14.13.39 FAD no flavin Homo sapiens
1.14.13.39 FMN no flavin Homo sapiens