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Literature summary extracted from
- N-Nitro-L-arginine and N-monomethyl-L-arginine exhibit a different pattern of inactivation toward the three nitric oxide synthases (1995), Arch. Biochem. Biophys., 320, 170-176.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | dithiothreitol | - |
Mus musculus |  |
| 1.14.13.39 | dithiothreitol | - |
Homo sapiens | |
| 1.14.13.39 | dithiothreitol | - |
Rattus norvegicus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | Ngamma-monomethyl-L-arginine | endothelial and neuronal isoforms: reversible inhibition; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Homo sapiens | |
| 1.14.13.39 | Ngamma-monomethyl-L-arginine | inducible isoform: after preincubation irreversible, time- and concentration-dependent inactivation, without preincubation reversible inhibition; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Mus musculus | |
| 1.14.13.39 | Ngamma-monomethyl-L-arginine | endothelial and neuronal isoforms: reversible inhibition; in presence of tetrahydrobiopterin 0.004 mM the neuronal isoform is inactivated; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Rattus norvegicus | |
| 1.14.13.39 | Ngamma-nitro-L-arginine | irreversible inactivation of neuronal and endothelial isoform after preincubation, unaffected by tetrahydrobiopterin; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Homo sapiens | |
| 1.14.13.39 | Ngamma-nitro-L-arginine | L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site; reversible inhibitor of inducible isoform from macrophage | Mus musculus | |
| 1.14.13.39 | Ngamma-nitro-L-arginine | irreversible inactivation of neuronal and endothelial isoform after preincubation, unaffected by tetrahydrobiopterin; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.39 | cytosol | - |
Mus musculus | 5829 | - |
| 1.14.13.39 | cytosol | - |
Homo sapiens | 5829 | - |
| 1.14.13.39 | cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | Ca2+ | - |
Mus musculus | |
| 1.14.13.39 | Ca2+ | - |
Homo sapiens | |
| 1.14.13.39 | Ca2+ | - |
Rattus norvegicus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | Homo sapiens | - |
- |
- |
| 1.14.13.39 | Mus musculus | - |
- |
- |
| 1.14.13.39 | Rattus norvegicus | - |
Sprague-Dawley | - |
| 1.14.13.39 | Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | brain | - |
Mus musculus | - |
| 1.14.13.39 | brain | - |
Homo sapiens | - |
| 1.14.13.39 | brain | - |
Rattus norvegicus | - |
| 1.14.13.39 | cerebellum | - |
Homo sapiens | - |
| 1.14.13.39 | cerebellum | - |
Rattus norvegicus | - |
| 1.14.13.39 | endothelium | - |
Mus musculus | - |
| 1.14.13.39 | endothelium | - |
Rattus norvegicus | - |
| 1.14.13.39 | endothelium | umbilical vein cells | Homo sapiens | - |
| 1.14.13.39 | macrophage | - |
Homo sapiens | - |
| 1.14.13.39 | macrophage | - |
Rattus norvegicus | - |
| 1.14.13.39 | macrophage | RAW 264.7 cells | Mus musculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.39 | 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
| 1.14.13.39 | 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
| 1.14.13.39 | 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
| 1.14.13.39 | 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus Sprague-Dawley | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.39 | e-NOS | endothelial isoform III | Homo sapiens |
| 1.14.13.39 | i-NOS | isoform II, inducible | Mus musculus |
| 1.14.13.39 | i-NOS | isoform II, inducible | Homo sapiens |
| 1.14.13.39 | i-NOS | isoform II, inducible | Rattus norvegicus |
| 1.14.13.39 | n-NOS | isoform I, neuronal enzyme | Homo sapiens |
| 1.14.13.39 | n-NOS | isoform I, neuronal enzyme | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 37 | - |
assay at | Mus musculus |
| 1.14.13.39 | 37 | - |
assay at | Homo sapiens |
| 1.14.13.39 | 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 7.4 | - |
assay at | Mus musculus |
| 1.14.13.39 | 7.4 | - |
assay at | Homo sapiens |
| 1.14.13.39 | 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | - |
Mus musculus | |
| 1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | - |
Homo sapiens | |
| 1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | - |
Rattus norvegicus | |
| 1.14.13.39 | Calmodulin | - |
Mus musculus | |
| 1.14.13.39 | Calmodulin | - |
Homo sapiens | |
| 1.14.13.39 | Calmodulin | - |
Rattus norvegicus | |
| 1.14.13.39 | NADPH | - |
Mus musculus | |
| 1.14.13.39 | NADPH | - |
Homo sapiens | |
| 1.14.13.39 | NADPH | - |
Rattus norvegicus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.39 | 0.00002 | - |
Ngamma-nitro-L-arginine | irreversible inactivation of endothelial isoform after preincubation | Homo sapiens | |
| 1.14.13.39 | 0.00009 | - |
Ngamma-nitro-L-arginine | irreversible inactivation of neuronal isoform after preincubation | Rattus norvegicus | |
| 1.14.13.39 | 0.0007 | - |
Ngamma-monomethyl-L-arginine | - |
Rattus norvegicus | |
| 1.14.13.39 | 0.0007 | - |
Ngamma-monomethyl-L-arginine | endothelial isoform | Homo sapiens | |
| 1.14.13.39 | 0.002 | - |
Ngamma-monomethyl-L-arginine | neuronal isoform in presence of 0.004 mM tetrahydrobiopterin | Rattus norvegicus | |
| 1.14.13.39 | 0.0026 | - |
Ngamma-monomethyl-L-arginine | inducible isoform from macrophage after preincubation | Mus musculus | |
| 1.14.13.39 | 0.0039 | - |
Ngamma-monomethyl-L-arginine | inducible isoform from macrophage without preincubation | Mus musculus | |
| 1.14.13.39 | 0.0065 | - |
Ngamma-monomethyl-L-arginine | neuronal isoform | Rattus norvegicus | |
| 1.14.13.39 | 0.0081 | - |
Ngamma-nitro-L-arginine | reversible inhibition of inducible macrophage isoform | Mus musculus | |
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