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Literature summary extracted from

  • Wever, R.M.F.; Van Dam, T.; Van Rijn, H.J.M.; De Groot, F.; Rabelink, T.J.
    Tetrahydrobiopterin regulates superoxide and nitric oxide generation by recombinant endothelial nitric oxide synthase (1997), Biochem. Biophys. Res. Commun., 237, 340-344.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 expression of endothelial isoform III in Spodoptera frugiperda cells via baculovirus infection Bos taurus

General Stability

EC Number General Stability Organism
1.14.13.39 tetrahydrobiopterin stabilizes dimeric form Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 diphenylene iodonium inhibition of superoxide production of recombinant isoform III Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ Bos taurus
-
 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information Bos taurus enzyme shows also superoxide formation activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 recombinant endothelial isoform III from insect cells Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 endothelium isoform III Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Bos taurus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 additional information enzyme shows also superoxide formation activity, uneffected by L-arginine, inhibited by tetrahydrobiopterin and diphenyleneiodonium Bos taurus ?
-
 ?
1.14.13.39 additional information enzyme shows also superoxide formation activity Bos taurus ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin stimulates Bos taurus
1.14.13.39 FAD major source of superoxide production in absence of tetrahydrobiopterin Bos taurus
1.14.13.39 NADPH
-
 Bos taurus