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Purification and properties of nitrogenase from the cyanobacterium, Anabaena cylindrica

Hallenbeck, P.C.; Kostel, P.J.; Benemann, J.R.; Eur. J. Biochem. 98, 275-284 (1979)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.6.1
additional information
-
additional information
-
Anabaena cylindrica
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
-
Anabaena cylindrica
1.18.6.1
Molybdenum
-
Anabaena cylindrica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.6.1
220000
-
MoFe protein, gel filtration
Anabaena cylindrica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
Anabaena cylindrica
-
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Anabaena cylindrica
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.6.1
Anabaena cylindrica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.18.6.1
-
Anabaena cylindrica
Reaction
EC Number
Reaction
Commentary
Organism
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Anabaena cylindrica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
additional information
-
-
Anabaena cylindrica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
440170
Anabaena cylindrica
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
-
-
?
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
440170
Anabaena cylindrica
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
440170
Anabaena cylindrica
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440170
Anabaena cylindrica
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440170
Anabaena cylindrica
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440170
Anabaena cylindrica
?
1.18.6.1
dithionite + H+ + N2 + ATP
in vitro substrate
440170
Anabaena cylindrica
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.18.6.1
More
-
Anabaena cylindrica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.6.1
additional information
-
additional information
-
Anabaena cylindrica
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
-
Anabaena cylindrica
1.18.6.1
Molybdenum
-
Anabaena cylindrica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.6.1
220000
-
MoFe protein, gel filtration
Anabaena cylindrica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
Anabaena cylindrica
-
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Anabaena cylindrica
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.6.1
-
Anabaena cylindrica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
additional information
-
-
Anabaena cylindrica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
440170
Anabaena cylindrica
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
-
-
?
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
440170
Anabaena cylindrica
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
440170
Anabaena cylindrica
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440170
Anabaena cylindrica
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440170
Anabaena cylindrica
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440170
Anabaena cylindrica
?
1.18.6.1
dithionite + H+ + N2 + ATP
in vitro substrate
440170
Anabaena cylindrica
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.18.6.1
More
-
Anabaena cylindrica