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Literature summary extracted from

  • Inouye, K.; Sakaki, T.
    Enzymatic studies on the key enzymes of vitamin D metabolism; 1alpha-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24) (2001), Biotechnol. Annu. Rev., 7, 179-194.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.18 expression in Escherichia coli Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.18 vitamin D3
-
 Homo sapiens
1.14.15.18 vitamin D3
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.18 0.0011
-
 (24R),25-dihydroxycholecalciferol
-
 Homo sapiens
1.14.15.18 0.0013
-
 24,25-dihydroxycholecalciferol
-
 Mus musculus
1.14.15.18 0.0027
-
 25-hydroxycholecalciferol
-
 Mus musculus
1.14.15.18 0.0027
-
 25-hydroxycholecalciferol
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.18 mitochondrion
-
 Mus musculus 5739
-
1.14.15.18 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.18 50000
-
 recombinant enzyme Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2 Mus musculus
-
 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2 Homo sapiens
-
 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 Mus musculus recombinant in E. coli calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 Homo sapiens recombinant in E. coli calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 Mus musculus central role in calcium regulation calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 Homo sapiens central role in calcium regulation calcitriol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.18 Homo sapiens
-
-
-
1.14.15.18 Mus musculus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstitution of the enzyme system in E. coli cells, consisting of NADH-adrenodoxin reductase, adrenodoxin and CYP27B1 Mus musculus
a
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstitution of the enzyme system in E. coli cells, consisting of NADH-adrenodoxin reductase, adrenodoxin and CYP27B1 Homo sapiens
a
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O cytochrome P-450 enzyme Mus musculus
a
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O cytochrome P-450 enzyme Homo sapiens
a
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase Mus musculus
a
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.18 kidney
-
 Mus musculus
-
1.14.15.18 kidney
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.18 0.0000002
-
-
 Homo sapiens
1.14.15.18 0.0000002
-
 substrate 23,25-dihydroxycholecalciferol Mus musculus
1.14.15.18 0.00000042
-
 substrate 24-oxo-25-hydroxycholecalciferol, recombinant reconstituted system Homo sapiens
1.14.15.18 0.00000058
-
 substrate 25-hydroxycholecalciferol, recombinant reconstituted system Homo sapiens
1.14.15.18 0.0000011
-
 substrate 24-oxo-25-hydroxycholecalciferol Mus musculus
1.14.15.18 0.0000015
-
-
 Mus musculus
1.14.15.18 0.0000015
-
 substrate 24,25-dihydroxycholecalciferol Homo sapiens
1.14.15.18 0.0000043
-
 substrate 24,25-dihydroxycholecalciferol Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2
-
 Mus musculus 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2
-
 Homo sapiens 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2 higher activity than with 25-hydroxycholecalciferol Mus musculus 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2 higher activity than with 25-hydroxycholecalciferol Homo sapiens 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 24,25-dihydroxycholecalciferol + reduced adrenodoxin + O2
-
 Mus musculus 1alpha,24,25-trihydroxycholecalciferol + oxidized adrenodoxin + H2O
-
 r
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 recombinant in E. coli Mus musculus calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 recombinant in E. coli Homo sapiens calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 central role in calcium regulation Mus musculus calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 central role in calcium regulation Homo sapiens calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Mus musculus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?
1.14.15.18 calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Homo sapiens calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?
1.14.15.18 additional information the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity Mus musculus ?
-
 ?
1.14.15.18 additional information the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.18 CYB27B1
-
 Mus musculus
1.14.15.18 CYB27B1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.18 NADPH
-
 Mus musculus
1.14.15.18 NADPH
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.15.18 0.001 0.002 vitamin D3
-
 Mus musculus
1.14.15.18 0.001 0.002 vitamin D3
-
 Homo sapiens