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Literature summary extracted from

  • Sawada, N.; Sakaki, T.; Kitanaka, S.; Takeyama, K.i.; Kato, S.; Inouye, K.
    Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase: coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli (1999), Eur. J. Biochem., 265, 950-956.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.18 expression in Escherichia coli JM109, coexpression of adrenodoxin and NADPH-adrenodoxin reductase for reconstitution of the system Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.18 G125E native mutant, no enzyme activity but normal expression level Homo sapiens
1.14.15.18 P382S native mutant, no enzyme activity but normal expression level Homo sapiens
1.14.15.18 R107H native mutant, no enzyme activity but normal expression level Homo sapiens
1.14.15.18 R335P native mutant, no enzyme activity but normal expression level Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.18 0.0011
-
 (24R),25-dihydroxycholecalciferol reconstituted system Homo sapiens
1.14.15.18 0.0027
-
 25-hydroxycholecalciferol reconstituted system Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 Homo sapiens
-
 calcitriol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.18 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase Homo sapiens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.18 0.00000042
-
 substrate 24-oxo-25-hydroxycholecalciferol, recombinant reconstituted system Homo sapiens
1.14.15.18 0.00000058
-
 substrate 25-hydroxycholecalciferol, recombinant reconstituted system Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2 higher activity than with 25-hydroxycholecalciferol Homo sapiens 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2 reconstituted system Homo sapiens 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 24-oxo-25-hydroxycholecalciferol + NADPH + O2 low activity, reconstituted system Homo sapiens ?
-
 ?
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2
-
 Homo sapiens calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Homo sapiens calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?
1.14.15.18 additional information not active with (23S),25-dihydroxycholecalciferol and 24-oxo-23,25-dihydroxycholecalciferol Homo sapiens ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.18 NADPH
-
 Homo sapiens