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Literature summary extracted from

  • Tumbula-Hansen, D.; Feng, L.; Toogood, H.; Stetter, K.O.; Söll, D.
    Evolutionary divergence of the archaeal aspartyl-tRNA synthetases into discriminating and nondiscriminating forms (2002), J. Biol. Chem., 277, 37184-37190.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.12 expression as His-tagged protein in Escherichia coli Methanothermobacter thermautotrophicus
6.1.1.12 expression as His-tagged protein in Escherichia coli Thermococcus kodakarensis
6.1.1.12 expression as His-tagged protein in Escherichia coli Ferroplasma acidarmanus
6.1.1.12 expression in Escherichia coli Thermococcus kodakarensis
6.1.1.12 expression in Escherichia coli Ferroplasma acidarmanus
6.1.1.23 expression in Escherichia coli Methanothermobacter thermautotrophicus
6.3.5.6 cloning of the gatCAB operon encoding the Asp-tRNAAsn amidotransferase and expression in Escherichia coli BL21-Codon Plus(DE3)-RIL Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsn Methanothermobacter thermautotrophicus the archaeal AspRS enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn, which is the intermediate in AsntRNAAsn generation by ASp-tRNAAsn amidotransferase, in contrary bacterial enzymes are discriminating ones AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp Methanothermobacter thermautotrophicus
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp Thermococcus kodakarensis
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp Ferroplasma acidarmanus
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 additional information Thermococcus kodakarensis no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase ?
-
 ?
6.1.1.12 additional information Ferroplasma acidarmanus no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase ?
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn Methanothermobacter thermautotrophicus
-
 AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn Methanothermobacter thermautotrophicus DSM 1053
-
 AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp Methanothermobacter thermautotrophicus
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp Methanothermobacter thermautotrophicus DSM 1053
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.3.5.6 Asp-tRNAAsn + ? Methanothermobacter thermautotrophicus the mischarging Asp-tRNA synthetase correlates with the absence in the genome of Asn-tRNA synthetase and the presence of Asp-tRNAAsn amidotransferase, employed by the transamidation pathway Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? Methanothermobacter thermautotrophicus enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Asn-tRNAAsn + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Ferroplasma acidarmanus
-
-
-
6.1.1.12 Methanothermobacter thermautotrophicus
-
-
-
6.1.1.12 Thermococcus kodakarensis Q52428
-
-
6.1.1.23 Methanothermobacter thermautotrophicus O26328
-
-
6.1.1.23 Methanothermobacter thermautotrophicus DSM 1053 O26328
-
-
6.3.5.6 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.12
-
 Thermococcus kodakarensis
6.1.1.12
-
 Ferroplasma acidarmanus
6.1.1.12 recombinant His-tagged enzyme from Escherichia coli Methanothermobacter thermautotrophicus
6.1.1.12 recombinant His-tagged enzyme from Escherichia coli Thermococcus kodakarensis
6.1.1.12 recombinant His-tagged enzyme from Escherichia coli Ferroplasma acidarmanus
6.1.1.23
-
 Methanothermobacter thermautotrophicus
6.3.5.6 purification of recombinant enzyme, expressed in Escherichia coli Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsn
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsn the archaeal AspRS enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn, which is the intermediate in AsntRNAAsn generation by ASp-tRNAAsn amidotransferase, in contrary bacterial enzymes are discriminating ones Methanothermobacter thermautotrophicus AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Thermococcus kodakarensis AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Ferroplasma acidarmanus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp no activity with tRNAAsn Thermococcus kodakarensis AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp no activity with tRNAAsn Ferroplasma acidarmanus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 additional information no acylation with L-aspartate of tRNAAsn Thermococcus kodakarensis ?
-
 ?
6.1.1.12 additional information no acylation with L-aspartate of tRNAAsn Ferroplasma acidarmanus ?
-
 ?
6.1.1.12 additional information no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase Thermococcus kodakarensis ?
-
 ?
6.1.1.12 additional information no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase Ferroplasma acidarmanus ?
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn
-
 Methanothermobacter thermautotrophicus DSM 1053 AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
 Methanothermobacter thermautotrophicus DSM 1053 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.3.5.6 Asp-tRNAAsn + ? the mischarging Asp-tRNA synthetase correlates with the absence in the genome of Asn-tRNA synthetase and the presence of Asp-tRNAAsn amidotransferase, employed by the transamidation pathway Methanothermobacter thermautotrophicus Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Methanothermobacter thermautotrophicus Asn-tRNAAsn + ?
-
 ?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
 Methanothermobacter thermautotrophicus ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.6 heterotrimer GatCAB enzyme Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.12 Aspartic acid translase
-
 Methanothermobacter thermautotrophicus
6.1.1.12 Aspartic acid translase
-
 Thermococcus kodakarensis
6.1.1.12 Aspartic acid translase
-
 Ferroplasma acidarmanus
6.1.1.12 Aspartyl ribonucleate synthetase
-
 Methanothermobacter thermautotrophicus
6.1.1.12 Aspartyl ribonucleate synthetase
-
 Thermococcus kodakarensis
6.1.1.12 Aspartyl ribonucleate synthetase
-
 Ferroplasma acidarmanus
6.1.1.12 aspartyl ribonuleic synthetase
-
 Methanothermobacter thermautotrophicus
6.1.1.12 aspartyl ribonuleic synthetase
-
 Thermococcus kodakarensis
6.1.1.12 aspartyl ribonuleic synthetase
-
 Ferroplasma acidarmanus
6.1.1.12 Aspartyl-transfer ribonucleic acid synthetase
-
 Methanothermobacter thermautotrophicus
6.1.1.12 Aspartyl-transfer ribonucleic acid synthetase
-
 Thermococcus kodakarensis
6.1.1.12 Aspartyl-transfer ribonucleic acid synthetase
-
 Ferroplasma acidarmanus
6.1.1.12 Aspartyl-transfer RNA synthetase
-
 Methanothermobacter thermautotrophicus
6.1.1.12 Aspartyl-transfer RNA synthetase
-
 Thermococcus kodakarensis
6.1.1.12 Aspartyl-transfer RNA synthetase
-
 Ferroplasma acidarmanus
6.1.1.12 Aspartyl-tRNA synthetase
-
 Methanothermobacter thermautotrophicus
6.1.1.12 Aspartyl-tRNA synthetase
-
 Thermococcus kodakarensis
6.1.1.12 Aspartyl-tRNA synthetase
-
 Ferroplasma acidarmanus
6.1.1.12 AspRS
-
 Methanothermobacter thermautotrophicus
6.1.1.12 discriminating aspartyl-tRNA synthetase
-
 Thermococcus kodakarensis
6.1.1.12 discriminating aspartyl-tRNA synthetase
-
 Ferroplasma acidarmanus
6.1.1.23 nondiscriminating aspartyl-tRNA synthetase
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.12 37
-
 assay at Ferroplasma acidarmanus
6.1.1.12 60
-
-
 Thermococcus kodakarensis
6.3.5.6 37
-
 assay at, as a compromise between optimal enzyme activity and the stability of the reaction product Methanothermobacter thermautotrophicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.12 37 60
-
 Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.12 6
-
-
 Thermococcus kodakarensis
6.1.1.12 6
-
-
 Ferroplasma acidarmanus
6.1.1.12 7
-
 assay at Methanothermobacter thermautotrophicus