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Literature summary extracted from

  • Itoh, H.; Hirota, A.; Hirayama, K.; Shin, T.; Murao, S.
    Properties of ascorbate oxidase produced by Acremonium sp. HI-25 (1995), Biosci. Biotechnol. Biochem., 59, 1052-1056.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.3 CuCl2 1 mM, 2fold activation Acremonium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.3 azide 1 mM, complete inhibition Acremonium
1.10.3.3 cyanide 1 mM, 57% inhibition Acremonium
1.10.3.3 Fe3+ 1 mM, 91% inhibition Acremonium
1.10.3.3 H2S 1 mM, 97% inhibition Acremonium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.3 0.29
-
 L-ascorbate
-
 Acremonium
1.10.3.3 0.47
-
 O2
-
 Acremonium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.3 copper
-
 Acremonium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.3 80000
-
-
 Acremonium

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.3 Acremonium
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.3 glycoprotein 11.2% carbohydrate Acremonium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.3 D-isoascorbic acid + O2
-
 Acremonium 2-dehydroisoascorbate + H2O
-
 ?
1.10.3.3 L-ascorbyl-6-palmitate + O2 36% of activity with L-ascorbate Acremonium L-dehydroascorbyl-6-palmitate
-
 ?
1.10.3.3 L-ascorbyl-6-stearate + O2 29% of activity with L-ascorbate Acremonium L-dehydroascorbyl-6-stearate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.3 45
-
-
 Acremonium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.3 60
-
 stable for 30 min Acremonium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.3 1130
-
 O2
-
 Acremonium
1.10.3.3 3110
-
 L-ascorbate
-
 Acremonium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.3 4
-
-
 Acremonium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.3 6 10 at 30°C Acremonium