BRENDA - Enzyme Database

Ascorbate peroxidase: a novel antioxidant enzyme in insects

Mathews, M.C.; Summers, C.B.; Felton, G.W.; Arch. Insect Biochem. Physiol. 34, 57-68 (1997)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.11
Hydroxyurea
26% inhibition at 1 mM
Helicoverpa zea
1.11.1.11
KCN
10% inhibition at 5 mM
Helicoverpa zea
1.11.1.11
p-Aminophenol
not inhibitory
Helicoverpa zea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.11
0.12
-
H2O2
-
Helicoverpa zea
1.11.1.11
0.14
-
ascorbate
-
Helicoverpa zea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.11
10000
-
partially purified enzyme, molecular weight cut-off centrifugation
Helicoverpa zea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.11
L-ascorbate + H2O2
Helicoverpa zea
the enzyme may be important in removing H2O2 and lipid peroxides in insects
dehydroascorbate + 2 H2O
-
Helicoverpa zea
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.11
Helicoverpa zea
-
larvae
-
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.11
partial, from whole body homogenate, using ammonium sulfate fractionation, column chromatography on Sephadex G-75 and isoelectric focusing. Second isolation of the enzyme: from regurgitant of actively feeding fifth instar organism, using centrifugation, a concentrator and isoelectric focusing
Helicoverpa zea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.11.1.11
fat body
55.6% relative activity to salivary gland
Helicoverpa zea
-
1.11.1.11
malpighian tubule
31.1% relative activity to salivary gland
Helicoverpa zea
-
1.11.1.11
midgut
20% relative activity to salivary gland
Helicoverpa zea
-
1.11.1.11
salivary gland
highest activity
Helicoverpa zea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.11.1.11
7.1
-
substrate: cumene hydroperoxide
Helicoverpa zea
1.11.1.11
19.2
-
substrate: tert-butyl hydroperoxide
Helicoverpa zea
1.11.1.11
20.9
-
substrate: H2O2
Helicoverpa zea
1.11.1.11
46.7
-
partially purified enzyme
Helicoverpa zea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.11
cysteine + H2O2
enzyme partially purified from whole body homogenate, 40% of the activity with L-ascorbate
439867
Helicoverpa zea
? + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
cytochrome c + H2O2
enzyme partially purified from whole body homogenate, 44% of the activity with L-ascorbate
439867
Helicoverpa zea
? + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
glutathione + H2O2
enzyme partially purified from whole body homogenate: 22% of the activity with L-ascorbate, enzyme partially purified from regurgitant: 0% relative activity to L-ascorbate, when assayed at the same concentration
439867
Helicoverpa zea
? + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
guaiacol + H2O2
no activity
439867
Helicoverpa zea
?
-
-
-
-
1.11.1.11
L-ascorbate + H2O2
tert-butyl hydroperoxide and cumene hydroperoxide also serve as electron acceptor
439867
Helicoverpa zea
dehydroascorbate + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
L-ascorbate + H2O2
L-ascorbate is the most effective natural electron donor
439867
Helicoverpa zea
dehydroascorbate + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
L-ascorbate + H2O2
the enzyme may be important in removing H2O2 and lipid peroxides in insects
439867
Helicoverpa zea
dehydroascorbate + 2 H2O
-
439867
Helicoverpa zea
?
1.11.1.11
L-ascorbic acid + cumene hydroperoxide
34% of the activity with H2O2
439867
Helicoverpa zea
dehydroascorbate + 1,1-dimethylbenzylalcohol + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
L-ascorbic acid + tert-butylhydroperoxide
92% of the activity with H2O2
439867
Helicoverpa zea
dehydroascorbate + tert-butylalcohol
-
439867
Helicoverpa zea
?
1.11.1.11
additional information
no activity with guaiacol
439867
Helicoverpa zea
?
-
-
-
-
1.11.1.11
NADPH + H2O2
enzyme partially purified from whole body homogenate: 93% of the activity with L-ascorbate, enzyme partially purified from regurgitant: 36% of the activity with L-ascorbate, when assayed at the same concentration
439867
Helicoverpa zea
? + H2O
-
439867
Helicoverpa zea
?
Subunits
EC Number
Subunits
Commentary
Organism
1.11.1.11
monomer
1 * 10000, SDS-PAGE, partially purified enzyme
Helicoverpa zea
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
1.11.1.11
100
-
complete loss of activity
Helicoverpa zea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.11
7
-
assay at
Helicoverpa zea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.11
additional information
the enzyme may not be a heme-peroxidase
Helicoverpa zea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.11
additional information
the enzyme may not be a heme-peroxidase
Helicoverpa zea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.11
Hydroxyurea
26% inhibition at 1 mM
Helicoverpa zea
1.11.1.11
KCN
10% inhibition at 5 mM
Helicoverpa zea
1.11.1.11
p-Aminophenol
not inhibitory
Helicoverpa zea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.11
0.12
-
H2O2
-
Helicoverpa zea
1.11.1.11
0.14
-
ascorbate
-
Helicoverpa zea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.11
10000
-
partially purified enzyme, molecular weight cut-off centrifugation
Helicoverpa zea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.11
L-ascorbate + H2O2
Helicoverpa zea
the enzyme may be important in removing H2O2 and lipid peroxides in insects
dehydroascorbate + 2 H2O
-
Helicoverpa zea
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.11
partial, from whole body homogenate, using ammonium sulfate fractionation, column chromatography on Sephadex G-75 and isoelectric focusing. Second isolation of the enzyme: from regurgitant of actively feeding fifth instar organism, using centrifugation, a concentrator and isoelectric focusing
Helicoverpa zea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.11.1.11
fat body
55.6% relative activity to salivary gland
Helicoverpa zea
-
1.11.1.11
malpighian tubule
31.1% relative activity to salivary gland
Helicoverpa zea
-
1.11.1.11
midgut
20% relative activity to salivary gland
Helicoverpa zea
-
1.11.1.11
salivary gland
highest activity
Helicoverpa zea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.11.1.11
7.1
-
substrate: cumene hydroperoxide
Helicoverpa zea
1.11.1.11
19.2
-
substrate: tert-butyl hydroperoxide
Helicoverpa zea
1.11.1.11
20.9
-
substrate: H2O2
Helicoverpa zea
1.11.1.11
46.7
-
partially purified enzyme
Helicoverpa zea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.11
cysteine + H2O2
enzyme partially purified from whole body homogenate, 40% of the activity with L-ascorbate
439867
Helicoverpa zea
? + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
cytochrome c + H2O2
enzyme partially purified from whole body homogenate, 44% of the activity with L-ascorbate
439867
Helicoverpa zea
? + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
glutathione + H2O2
enzyme partially purified from whole body homogenate: 22% of the activity with L-ascorbate, enzyme partially purified from regurgitant: 0% relative activity to L-ascorbate, when assayed at the same concentration
439867
Helicoverpa zea
? + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
guaiacol + H2O2
no activity
439867
Helicoverpa zea
?
-
-
-
-
1.11.1.11
L-ascorbate + H2O2
tert-butyl hydroperoxide and cumene hydroperoxide also serve as electron acceptor
439867
Helicoverpa zea
dehydroascorbate + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
L-ascorbate + H2O2
L-ascorbate is the most effective natural electron donor
439867
Helicoverpa zea
dehydroascorbate + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
L-ascorbate + H2O2
the enzyme may be important in removing H2O2 and lipid peroxides in insects
439867
Helicoverpa zea
dehydroascorbate + 2 H2O
-
439867
Helicoverpa zea
?
1.11.1.11
L-ascorbic acid + cumene hydroperoxide
34% of the activity with H2O2
439867
Helicoverpa zea
dehydroascorbate + 1,1-dimethylbenzylalcohol + H2O
-
439867
Helicoverpa zea
?
1.11.1.11
L-ascorbic acid + tert-butylhydroperoxide
92% of the activity with H2O2
439867
Helicoverpa zea
dehydroascorbate + tert-butylalcohol
-
439867
Helicoverpa zea
?
1.11.1.11
additional information
no activity with guaiacol
439867
Helicoverpa zea
?
-
-
-
-
1.11.1.11
NADPH + H2O2
enzyme partially purified from whole body homogenate: 93% of the activity with L-ascorbate, enzyme partially purified from regurgitant: 36% of the activity with L-ascorbate, when assayed at the same concentration
439867
Helicoverpa zea
? + H2O
-
439867
Helicoverpa zea
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.11.1.11
monomer
1 * 10000, SDS-PAGE, partially purified enzyme
Helicoverpa zea
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
1.11.1.11
100
-
complete loss of activity
Helicoverpa zea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.11
7
-
assay at
Helicoverpa zea