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Literature summary extracted from

  • Paszczynski, A.; Crawford, R.L.; Huynh, V.B.
    Manganese peroxidase from Phanerochaete chrysosporium: Purification (1988), Methods Enzymol., 161, 264-270.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.13 H2O2 H2O2-dependent Phanerodontia chrysosporium
1.11.1.13 L-Tartrate activates, forms stable Mn3+ complex Phanerodontia chrysosporium

General Stability

EC Number General Stability Organism
1.11.1.13 stable to lyophilization Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.13 Co2+
-
 Phanerodontia chrysosporium
1.11.1.13 Cu2+
-
 Phanerodontia chrysosporium
1.11.1.13 Fe2+
-
 Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.13 extracellular
-
 Phanerodontia chrysosporium
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2 Phanerodontia chrysosporium involved in lignin-degradation Mn3+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Phanerodontia chrysosporium
-
-
-
1.11.1.13 Phanerodontia chrysosporium
-
 white rot basidomycete
-
1.11.1.13 Phanerodontia chrysosporium BKM-F 1767
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13 11.1fold purification Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.13 100
-
-
 Phanerodontia chrysosporium

Storage Stability

EC Number Storage Stability Organism
1.11.1.13 -20°C, freeze-dried preparation, up to a year, stable Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes curcumin ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes vanillylacetone ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes o-dianisidine ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes amines ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes guaiacol ?
1.11.1.13 Mn2+ + H+ + H2O2 involved in lignin-degradation Phanerodontia chrysosporium Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium BKM-F 1767 Mn3+ + H2O Mn3+ oxidizes curcumin ?
1.11.1.13 additional information in absence of H2O2 the enzyme shows Mn-dependent oxidase activity against glutathione, dithiothreitol and dihydroxymaleic acid, forming H2O2 at the expense of oxygen Phanerodontia chrysosporium ?
-
 ?
1.11.1.13 additional information in presence of H2O2 and Mn2+ the enzyme oxidizes a variety of phenolic compounds, especially vinyl and syringyl side-chain substituted substrates Phanerodontia chrysosporium ?
-
 ?
1.11.1.13 additional information in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ Phanerodontia chrysosporium ?
-
 ?
1.11.1.13 additional information in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+ Phanerodontia chrysosporium ?
-
 ?
1.11.1.13 additional information in absence of H2O2 the enzyme shows Mn-dependent oxidase activity against glutathione, dithiothreitol and dihydroxymaleic acid, forming H2O2 at the expense of oxygen Phanerodontia chrysosporium BKM-F 1767 ?
-
 ?
1.11.1.13 additional information in presence of H2O2 and Mn2+ the enzyme oxidizes a variety of phenolic compounds, especially vinyl and syringyl side-chain substituted substrates Phanerodontia chrysosporium BKM-F 1767 ?
-
 ?
1.11.1.13 additional information in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ Phanerodontia chrysosporium BKM-F 1767 ?
-
 ?
1.11.1.13 additional information in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+ Phanerodontia chrysosporium BKM-F 1767 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.13 22
-
 assay at Phanerodontia chrysosporium