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Literature summary extracted from

  • Brown-Peterson, N.J.; Salin, M.L.
    Purification and characterization of a mesohalic catalase from the halophilic bacterium Halobacterium halobium (1995), J. Bacteriol., 177, 378-384.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.11.1.6 protein must be purified in the presence of 2 M NaCl, although its highest activity is observed in the absence of salt Halobacterium salinarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-amino-1H-1,2,4-triazole 33% inactivation at 20 mM in 2.5 h Halobacterium salinarum
1.11.1.6 CaCl2 irreversible loss of activity at 2 M Halobacterium salinarum
1.11.1.6 KCN
-
 Halobacterium salinarum
1.11.1.6 MgCl2 irreversible loss of activity at 2 M Halobacterium salinarum
1.11.1.6 NaN3
-
 Halobacterium salinarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 60
-
 H2O2
-
 Halobacterium salinarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 62000
-
 4 * 62000, SDS-PAGE Halobacterium salinarum
1.11.1.6 240000
-
 gel filtration Halobacterium salinarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Halobacterium salinarum monofunctional catalase O2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Halobacterium salinarum
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.11.1.6 not sensitive to oxidation by singlet oxygen Halobacterium salinarum

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.6 glycoprotein
-
 Halobacterium salinarum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 to homogeneity, chromatography steps Halobacterium salinarum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 cell culture inducible at low salt concentrations Halobacterium salinarum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 6422
-
-
 Halobacterium salinarum

Storage Stability

EC Number Storage Stability Organism
1.11.1.6 4°C, 2 M NaCl, 1 month Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2 monofunctional catalase Halobacterium salinarum O2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer 4 * 62000, SDS-PAGE Halobacterium salinarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 45
-
 stable up to Halobacterium salinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 7
-
-
 Halobacterium salinarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 6.5 10 high activity within Halobacterium salinarum

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme 0.78 protoheme per tetramer Halobacterium salinarum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.6 0.002
-
 NaN3
-
 Halobacterium salinarum
1.11.1.6 0.018
-
 KCN
-
 Halobacterium salinarum