BRENDA - Enzyme Database show

Purification and properties of hydrogenase from Megasphaera elsdenii

Van Dijk, C.; Mayhew, S.G.; Grande, H.J.; Veeger, C.; Eur. J. Biochem. 102, 317-330 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.12.7.2
MgCl2
up to 0.5 M, activation of methyl or benzyl viologen mediated hydrogen oxidation
Megasphaera elsdenii
1.12.7.2
NaCl
up to 0.5 M, activation of methyl or benzyl viologen mediated hydrogen oxidation
Megasphaera elsdenii
1.12.7.2
Tris-HCl
at pH 8, activation of methyl or benzyl viologen mediated hydrogen oxidation
Megasphaera elsdenii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.7.2
5.7
-
benzyl viologen
hydrogen oxidation
Megasphaera elsdenii
1.12.7.2
171
-
methyl viologen
hydrogen oxidation
Megasphaera elsdenii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.7.2
49000
-
gel filtration
Megasphaera elsdenii
1.12.7.2
52000
-
SDS-PAGE
Megasphaera elsdenii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
Megasphaera elsdenii
-
H+ + reduced ferredoxin
-
Megasphaera elsdenii
r
1.12.7.2
H2 + oxidized ferredoxin
Megasphaera elsdenii LC1
-
H+ + reduced ferredoxin
-
Megasphaera elsdenii LC1
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.7.2
Megasphaera elsdenii
-
formerly Peptostreptococcus elsdenii
-
1.12.7.2
Megasphaera elsdenii LC1
-
formerly Peptostreptococcus elsdenii
-
Purification (Commentary)
EC Number
Commentary
Organism
1.12.7.2
-
Megasphaera elsdenii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.7.2
510
-
reaction with methyl viologen
Megasphaera elsdenii
1.12.7.2
1470
-
after addition of 1 M NaCl, reaction with methyl viologen
Megasphaera elsdenii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439677
Megasphaera elsdenii
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439677
Megasphaera elsdenii
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439677
Megasphaera elsdenii LC1
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439677
Megasphaera elsdenii LC1
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + oxidized ferredoxin
-
439677
Megasphaera elsdenii
H+ + reduced ferredoxin
-
439677
Megasphaera elsdenii
r
1.12.7.2
H2 + oxidized ferredoxin
-
439677
Megasphaera elsdenii LC1
H+ + reduced ferredoxin
-
439677
Megasphaera elsdenii LC1
r
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.12.7.2
MgCl2
up to 0.5 M, activation of methyl or benzyl viologen mediated hydrogen oxidation
Megasphaera elsdenii
1.12.7.2
NaCl
up to 0.5 M, activation of methyl or benzyl viologen mediated hydrogen oxidation
Megasphaera elsdenii
1.12.7.2
Tris-HCl
at pH 8, activation of methyl or benzyl viologen mediated hydrogen oxidation
Megasphaera elsdenii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.7.2
5.7
-
benzyl viologen
hydrogen oxidation
Megasphaera elsdenii
1.12.7.2
171
-
methyl viologen
hydrogen oxidation
Megasphaera elsdenii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.7.2
49000
-
gel filtration
Megasphaera elsdenii
1.12.7.2
52000
-
SDS-PAGE
Megasphaera elsdenii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
Megasphaera elsdenii
-
H+ + reduced ferredoxin
-
Megasphaera elsdenii
r
1.12.7.2
H2 + oxidized ferredoxin
Megasphaera elsdenii LC1
-
H+ + reduced ferredoxin
-
Megasphaera elsdenii LC1
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.7.2
-
Megasphaera elsdenii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.7.2
510
-
reaction with methyl viologen
Megasphaera elsdenii
1.12.7.2
1470
-
after addition of 1 M NaCl, reaction with methyl viologen
Megasphaera elsdenii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439677
Megasphaera elsdenii
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439677
Megasphaera elsdenii
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439677
Megasphaera elsdenii LC1
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439677
Megasphaera elsdenii LC1
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + oxidized ferredoxin
-
439677
Megasphaera elsdenii
H+ + reduced ferredoxin
-
439677
Megasphaera elsdenii
r
1.12.7.2
H2 + oxidized ferredoxin
-
439677
Megasphaera elsdenii LC1
H+ + reduced ferredoxin
-
439677
Megasphaera elsdenii LC1
r