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Literature summary extracted from

  • Miller, M.A.; Lipscomb, J.D.
    Homoprotocatechuate 2,3-dioxygenase from Brevibacterium fuscum. A dioxygenase with catalase activity (1996), J. Biol. Chem., 271, 5524-5535.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.15 H2O2 stable to H2O2, due to the fact that the enzyme is also an effective catalase Brevibacterium fuscum
1.13.11.15 K3Fe(CN)6 10 mM, 35% inhibition Brevibacterium fuscum
1.13.11.15 additional information stable to H2O2, due to the fact that the enzyme is also an effective catalase Brevibacterium fuscum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.15 0.015
-
 3,4-Dihydroxyphenylacetate
-
 Brevibacterium fuscum
1.13.11.15 0.06
-
 O2
-
 Brevibacterium fuscum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.15 Iron Fe(II)-containing, contains 0.9 gatom of iron per subunit Brevibacterium fuscum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.15 42500
-
 4 * 42500, or 6 * 42500, SDS-PAGE Brevibacterium fuscum
1.13.11.15 42500
-
 6 * 42500, or 42500, SDS-PAGE Brevibacterium fuscum
1.13.11.15 220000
-
 equilibrium sedimentation Brevibacterium fuscum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Brevibacterium fuscum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.15
-
 Brevibacterium fuscum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.15 additional information
-
-
 Brevibacterium fuscum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Brevibacterium fuscum 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 additional information enzyme also has catalase activity Brevibacterium fuscum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.15 hexamer 6 * 42500, or 42500, SDS-PAGE Brevibacterium fuscum
1.13.11.15 tetramer 4 * 42500, or 6 * 42500, SDS-PAGE Brevibacterium fuscum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.15 additional information
-
 additional information
-
 Brevibacterium fuscum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.15 8.5
-
-
 Brevibacterium fuscum