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Literature summary extracted from

  • Ono-Kamimoto, M.
    Studies on 3,4-dihydroxyphenylacetate 2,3-dioxygenase. I. Role of iron, substrate binding, and some other properties (1973), J. Biochem., 74, 1049-1059.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.15 Fe3+
-
 Pseudomonas putida
1.13.11.15 H2O2 strong inactivation Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.15 Iron 4-5 gatom of non-heme iron per mol of protein Pseudomonas putida
1.13.11.15 Iron the non-heme bound-iron is in the ferrous state and is essential for activity Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.15 35000
-
 4 * 35000, SDS-PAGE Pseudomonas putida
1.13.11.15 138000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxybenzoic acid + O2 0.125% of the activity with 3,4-dihydroxyphenylacetate Pseudomonas putida 2-hydroxy-5-carboxymuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Pseudomonas putida 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylpropionate + O2 2.86% of the activity with 3,4-dihydroxyphenylacetate Pseudomonas putida 2-hydroxy-5-carboxyethylmuconate semialdehyde
-
 ?
1.13.11.15 3-(3,4-dihydroxyphenyl)-DL-alanine + O2
-
 Pseudomonas putida (2E,4Z)-7-amino-5-formyl-2-hydroxyocta-2,4-dienedioic acid
-
 ?
1.13.11.15 additional information very low activity with various 4-substituted catechols Pseudomonas putida ?
-
 ?
1.13.11.15 N-formyl-3-(3,4-dihydroxyphenyl)-DL-alanine + O2
-
 Pseudomonas putida (2E,4Z)-5-formyl-7-(formylamino)-2-hydroxyocta-2,4-dienedioic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.15 tetramer 4 * 35000, SDS-PAGE Pseudomonas putida