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Literature summary extracted from

  • Camoretti-Mercado, B.; Frydman, R.B.
    Separation of tryptophan pyrrolooxygenase into three molecular forms. A study of their substrate specificities using tryptophyl-containing peptides and proteins (1986), Eur. J. Biochem., 156, 317-325.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.26 2-mercaptoethanol 2 mM, total activity loss Triticum aestivum
1.13.11.26 alpha,alpha'-dipyridyl
-
 Triticum aestivum
1.13.11.26 dithioerythritol 2 mM, total activity loss Triticum aestivum
1.13.11.26 dithiothreitol 2 mM, total activity loss Triticum aestivum
1.13.11.26 EDTA
-
 Triticum aestivum
1.13.11.26 o-phenanthroline
-
 Triticum aestivum
1.13.11.26 p-hydroxymercurisulfonate 10 mM, activity loss of 60% Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.26 25000
-
 molecular form A, SDS-PAGE Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.26 L-tryptophan + O2 Triticum aestivum
-
 formylkynurenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.26 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.26 three molecular forms: A,B,C Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.26 germ
-
 Triticum aestivum
-

Storage Stability

EC Number Storage Stability Organism
1.13.11.26 4°C, forms A and C stable for at least two weeks, form B activity loss after three days Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.26 D-tryptophan + O2
-
 Triticum aestivum formylkynurenine
-
 ?
1.13.11.26 DL-5-fluorotryptophan + O2
-
 Triticum aestivum 5-fluoroformylkynurenine
-
 ?
1.13.11.26 DL-5-methyltryptophan + O2
-
 Triticum aestivum 5-methyl-N-formylkynurenine
-
 ?
1.13.11.26 DL-6-fluorotryptophan + O2
-
 Triticum aestivum 4-fluoro-N-formylkynurenine
-
 ?
1.13.11.26 DL-6-methyltryptophan + O2
-
 Triticum aestivum 4-methyl-N-formylkynurenine
-
 ?
1.13.11.26 DL-7-methyltryptophan + O2
-
 Triticum aestivum 3-methyl-N-formylkynurenine
-
 ?
1.13.11.26 DL-tryptophan + O2
-
 Triticum aestivum formylkynurenine
-
 ?
1.13.11.26 DL-tryptophan methyl ester + O2
-
 Triticum aestivum formylkynurenine methyl ester
-
 ?
1.13.11.26 L-5-hydroxytryptophan + O2 very poor substrate activity with molecular form A Triticum aestivum 5-hydroxy-N-formylkynurenine
-
 ?
1.13.11.26 L-tryptophan + O2
-
 Triticum aestivum formylkynurenine
-
 ?
1.13.11.26 N-acetyl-5-methyltryptophan + O2
-
 Triticum aestivum 5-methyl-Nalpha-acetyl-Nomega-formylkynurenine
-
 ?
1.13.11.26 N-acetyl-DL-tryptophan + O2
-
 Triticum aestivum N-acetylformylkynurenine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.26 22
-
 form C with chymotrypsin as substrate Triticum aestivum
1.13.11.26 37
-
 form B, form C with tryptophan as substrate Triticum aestivum
1.13.11.26 37 45 form A, tryptophan or gamma-chymotrypsin as substrates Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.26 8.6
-
 in Tris-HCl buffer Triticum aestivum