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Literature summary extracted from

  • Ochi, K.; Yoshimoto, Y.; Yamamoto, S.; Taniguchi, K.; Miyamoto, T.
    Archidonate 5-lipoxygenase of guinea pig peritoneal polymorphonuclear leukocytes (1983), J. Biol. Chem., 258, 5754-5758.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.34 5,8,11,14,17-eicosapentaenoic acid competitive inhibition of oxygenation of arachidonic acid Cavia porcellus
1.13.11.34 adenosine slight stimulation Cavia porcellus
1.13.11.34 ADP stimulation Cavia porcellus
1.13.11.34 AMP stimulation Cavia porcellus
1.13.11.34 ATP stimulates Cavia porcellus
1.13.11.34 cAMP stimulation Cavia porcellus
1.13.11.34 GTP stimulation Cavia porcellus
1.13.11.34 UTP stimulation Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.34 5,8,11,14,17-eicosapentaenoic acid
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.34 0.013
-
 5,8,11,14,17-eicosapentaenoic acid
-
 Cavia porcellus
1.13.11.34 0.019
-
 arachidonic acid
-
 Cavia porcellus
1.13.11.34 0.09
-
 8,11,14-eicosatrienoic acid
-
 Cavia porcellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.34 Ca2+ required Cavia porcellus
1.13.11.34 Ca2+ half-maximal activity at 0.02 mM Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.34 partial Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.34 leukocyte peritoneal polymorphonuclear Cavia porcellus
-

Storage Stability

EC Number Storage Stability Organism
1.13.11.34 -70°C, 1 month, 50% loss of activity Cavia porcellus
1.13.11.34 0°C, 30 h, about 70% loss of activity, in presence of 2 mM ATP only 30% loss of activity Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 5,8,11,14,17-eicosapentaenoic acid + O2
-
 Cavia porcellus ?
-
 ?
1.13.11.34 8,11,14-eicosatrienoic acid + O2
-
 Cavia porcellus ?
-
 ?
1.13.11.34 arachidonate + O2 i.e. 5,8,11,14-eicosatetraenoic acid Cavia porcellus (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.34 40
-
 5 min, stable up to Cavia porcellus
1.13.11.34 60
-
 5 min, most of the activity is lost Cavia porcellus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.34 0.025
-
 5,8,11,14,17-eicosapentaenoic acid
-
 Cavia porcellus