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Literature summary extracted from

  • Koontz, W.A.; Shiman, R.
    Beef kidney 3-hydroxyanthranilic acid oxygenase. Purification, characterization, and analysis of the assay (1976), J. Biol. Chem., 251, 368-377.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.6 Fe2+ one Fe2+ per active site Bos taurus
1.13.11.6 Fe3+ does not seem to bind to the enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.6 34000
-
 gel filtration, readily aggregates to form inactive higher molecular weight oligomers Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.6
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.6 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.6 140
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.13.11.6 -90°C, frozen in dry ice-ethanol bath, partially purified enzyme is stable for at least 1 month, purified enzyme is unstable Bos taurus
1.13.11.6 0°C, 0.01 M collidine chloride, 0.01 M potassium chloride, pH 6.5, about 15% loss of activity after 1 month, purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.6 3-hydroxyanthranilate + O2
-
 Bos taurus 2-amino-3-carboxymuconate semialdehyde
-
 ?