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Literature summary extracted from

  • Kondo, A.; Blanchard, J.S.; Englard, S.
    Purification and properties of calf liver gamma-butyrobetaine hydroxylase (1981), Arch. Biochem. Biophys., 212, 338-346.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.1 catalase
-
 Bos taurus

General Stability

EC Number General Stability Organism
1.14.11.1 dialysis, 4°C, 50% loss of activity overnight Bos taurus
1.14.11.1 ultra-filtration, 4°C, 50% loss of activity overnight Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.1 alpha,alpha'-bipyridyl no activity at 2 mM Bos taurus
1.14.11.1 Ba2+
-
 Bos taurus
1.14.11.1 Ca2+
-
 Bos taurus
1.14.11.1 Cd2+
-
 Bos taurus
1.14.11.1 Co2+
-
 Bos taurus
1.14.11.1 Cu2+
-
 Bos taurus
1.14.11.1 dioxane
-
 Bos taurus
1.14.11.1 Hg2+
-
 Bos taurus
1.14.11.1 Mg2+
-
 Bos taurus
1.14.11.1 Mn2+
-
 Bos taurus
1.14.11.1 Ni2+
-
 Bos taurus
1.14.11.1 Pb2+
-
 Bos taurus
1.14.11.1 Sn2+
-
 Bos taurus
1.14.11.1 Zn2+
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.1 additional information
-
 additional information
-
 Bos taurus
1.14.11.1 0.012
-
 Fe2+
-
 Bos taurus
1.14.11.1 0.51
-
 gamma-butyrobetaine
-
 Bos taurus
1.14.11.1 0.82
-
 2-oxoglutarate
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.1 cytoplasm
-
 Bos taurus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.1 Fe2+ requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.1 42000
-
 2 * 42000, SDS-PAGE Bos taurus
1.14.11.1 46000
-
 2 * 46000, SDS-PAGE Bos taurus
1.14.11.1 80000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.1 4-trimethylammoniobutanoate + 2-oxoglutarate + O2 Bos taurus gamma-butyrobetaine, terminal reaction in the pathway of L-carnitine biosynthesis 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.14.11.1 Acetone powder, retains full activity of fresh liver homogenate Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.1 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.1
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.1 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.1 0.053
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.14.11.1 -90°C, stable for a year or more as acetone powder, stable for 3 months as purified and lyophilized enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.1 4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
 Bos taurus 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
 ?
1.14.11.1 4-trimethylammoniobutanoate + 2-oxoglutarate + O2 gamma-butyrobetaine, terminal reaction in the pathway of L-carnitine biosynthesis Bos taurus 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.1 dimer 2 * 42000, SDS-PAGE Bos taurus
1.14.11.1 dimer 2 * 46000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.1 37
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.1 ascorbate
-
 Bos taurus