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Literature summary extracted from

  • Lindstedt, G.; Lindstedt, S.; Nordin, I.
    Purification and properties of gamma-butyrobetaine hydroxylase from Pseudomonas sp AK 1 (1977), Biochemistry, 16, 2181-2188.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.1 cytoplasm
-
 Pseudomonas sp. 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.1 Fe2+ requirement Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.1 37000
-
 1 * 39000 + 1 * 37000, SDS-PAGE, amino acid analysis, N-terminal amino acid sequence Pseudomonas sp.
1.14.11.1 39000
-
 1 * 39000 + 1 * 37000, SDS-PAGE, amino acid analysis, N-terminal amino acid sequence Pseudomonas sp.
1.14.11.1 86000
-
 gel filtration Pseudomonas sp.
1.14.11.1 86000 95000 gel filtration, sedimentation equilibrium centrifugation Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.1 Pseudomonas sp.
-
 AK1
-
1.14.11.1 Pseudomonas sp. AK1
-
 AK1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.1 21.6
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.14.11.1 -20°C to 4°C, 50% loss of activity in a week Pseudomonas sp.
1.14.11.1 -60° to -170°C, stable for 6 months in potassium phosphate buffer, pH 6.5 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.1 4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
 Pseudomonas sp. 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
 ?
1.14.11.1 4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
 Pseudomonas sp. AK1 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.1 dimer 1 * 39000 + 1 * 37000, SDS-PAGE, amino acid analysis, N-terminal amino acid sequence Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.1 37
-
 assay at Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.1 34
-
 gamma-butyrobetaine
-
 Pseudomonas sp.