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Literature summary extracted from

  • Kwak, S.S.; Kamiya, Y.; Sakurai, A.; Takahashi, N.; Graebe, J.
    Partial purification and characterization of gibberellin 3beta-hydroxylase from immature seeds of Phaseolus vulgaris (1988), Plant Cell Physiol., 29, 935-943.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.15 bovine serum albumin 2 mg/ml, 30% activation of purified enzyme, stimulation by bovine serum albumin and catalase is additive and less pronounced in crude cell extract Phaseolus vulgaris
1.14.11.15 catalase 0.5 mg/ml, 30% activation of purified enzyme, stimulation by catalase and bovine serum albumin is additive and less pronounced in crude cell extract Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.15 2,2'-bipyridyl
-
 Phaseolus vulgaris
1.14.11.15 Cd2+ 0.5 mM, strong Phaseolus vulgaris
1.14.11.15 Co2+ 0.5 mM, strong Phaseolus vulgaris
1.14.11.15 Cu2+ 0.5 mM, strong Phaseolus vulgaris
1.14.11.15 gibberellin a15 inhibits 3beta-hydroxylation of gibberellin a20 Phaseolus vulgaris
1.14.11.15 gibberellin a20
-
 Phaseolus vulgaris
1.14.11.15 gibberellin a44 inhibits 3beta-hydroxylation of gibberellin a20 Phaseolus vulgaris
1.14.11.15 gibberellin a5 inhibits 3beta-hydroxylation of gibberellin a20 Phaseolus vulgaris
1.14.11.15 gibberellin a9 inhibits 3beta-hydroxylation of gibberellin a20 Phaseolus vulgaris
1.14.11.15 Hg2+ 0.5 mM, strong Phaseolus vulgaris
1.14.11.15 Mn2+ 0.5 mM, strong Phaseolus vulgaris
1.14.11.15 Ni2+ 0.5 mM, strong Phaseolus vulgaris
1.14.11.15 Zn2+ 0.5 mM, strong Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.15 0.00029
-
 gibberellin a20
-
 Phaseolus vulgaris
1.14.11.15 0.00033
-
 gibberellin a9
-
 Phaseolus vulgaris
1.14.11.15 0.25
-
 2-oxoglutarate with gibberellin a20 as cosubstrate Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.15 Fe2+ activates, at least 0.2 mM for full activation Phaseolus vulgaris
1.14.11.15 Fe3+ activation in the presence of ascorbate Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.15 42000
-
 gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.15 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.15 partial Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.15 seed immature Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.15 additional information
-
-
 Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.15 gibberellin a20 + 2-oxoglutarate + O2
-
 Phaseolus vulgaris gibberellin a1 + succinate + CO2
-
 ?
1.14.11.15 gibberellin a9 + 2-oxoglutarate + O2
-
 Phaseolus vulgaris gibberellin a4 + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.15 monomer 1 * 42000, SDS-PAGE Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.15 7.7
-
-
 Phaseolus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.11.15 7 9 about half-maximal activity at pH 7.0 and pH 9.0 Phaseolus vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.15 ascorbate 5 mM, 10fold stimulation Phaseolus vulgaris