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Literature summary extracted from

  • Baader, E.; Tschank, G.; Baringhaus, K.H.; Burghard, H.; Guenzler, V.
    Inhibition of prolyl 4-hydroxylase by oxalyl amino acid derivatives in vitro, in isolated microsomes and in embryonic chicken tissues (1994), Biochem. J., 300, 525-530.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 dithiothreitol activation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 dimethyloxalylalanine 50% inhibition in chicken embryo calvaria at 1 mM Gallus gallus
1.14.11.2 dimethyloxalylglycine 50% inhibition in chicken embryo calvaria at 0.002 mM, inhibitor of hydroxyproline synthesis in embryonic chicken lung Gallus gallus
1.14.11.2 oxalyl-beta-alanine competitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 oxalylalanine inhibits purified enzyme, competitive inhibition with respect to 2-oxoglutarate, 50% inhibition of microsomal enzyme at 0.123 mM Gallus gallus
1.14.11.2 oxalylcystine competitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 oxalylglycine inhibits purified enzyme, competitive inhibition with respect to 2-oxoglutarate, 50% inhibition of microsomal enzyme at 0.023 mM Gallus gallus
1.14.11.2 oxalylproline
-
 Gallus gallus
1.14.11.2 oxalylsarcosine noncompetitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 oxalylvaline
-
 Gallus gallus
1.14.11.2 Pyridine 2,4-dicarboxylate
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.2 microsome embryonic bone Gallus gallus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 using ammonium sulfate fractionation, affinity chromatography on poly-L-proline coupled to Sepharose 4B and DEAE-cellulose chromatography Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 bone embryo Gallus gallus
-
1.14.11.2 calvarium embryo Gallus gallus
-
1.14.11.2 embryo
-
 Gallus gallus
-
1.14.11.2 lung embryo Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
1.14.11.2 additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
 assay at Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate
-
 Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.0019 0.007 oxalylglycine
-
 Gallus gallus
1.14.11.2 0.04
-
 oxalylalanine
-
 Gallus gallus
1.14.11.2 1
-
 oxalylsarcosine
-
 Gallus gallus
1.14.11.2 1.25
-
 oxalylcystine
-
 Gallus gallus
1.14.11.2 1.4
-
 oxalyl-beta-alanine
-
 Gallus gallus
1.14.11.2 10
-
 oxalylproline value above Gallus gallus
1.14.11.2 10
-
 oxalylvaline value above Gallus gallus