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Literature summary extracted from

  • Bolwell, G.P.; Robbins, M.P.; Dixon, R.A.
    Elicitor-induced prolyl hydroxylase from French bean (Phaseolus vulgaris). Localization, purification and properties (1985), Biochem. J., 229, 693-699.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 Triton X-100 activation at 0.1% v/v Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 2,2'-dipyridyl
-
 Phaseolus vulgaris
1.14.11.2 EDTA
-
 Phaseolus vulgaris
1.14.11.2 EGTA
-
 Phaseolus vulgaris
1.14.11.2 Salicylyl hydroxamate
-
 Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.005
-
 poly(L-Pro) MW 30000 Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.2 endoplasmic reticulum
-
 Phaseolus vulgaris 5783
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 65000
-
 x * 65000, SDS-PAGE Phaseolus vulgaris
1.14.11.2 300000
-
 gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Phaseolus vulgaris
-
 french bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 using ion-exchange chromatography on DEAE-cellulose and affinity chromatography on a column of poly-L-proline-Sepharose 4B Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 cell suspension culture
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.2 additional information
-
-
 Phaseolus vulgaris
1.14.11.2 0.0714
-
 homogenate Phaseolus vulgaris
1.14.11.2 0.138
-
 15000 g pellet Phaseolus vulgaris
1.14.11.2 0.162
-
 microsomal fraction, rough membrane Phaseolus vulgaris
1.14.11.2 0.27
-
 1000 g pellet Phaseolus vulgaris
1.14.11.2 0.63
-
 microsomal fraction Phaseolus vulgaris
1.14.11.2 1.668
-
 microsomal fraction, smooth membrane Phaseolus vulgaris
1.14.11.2 2.328
-
 purified enzyme, 6 M-urea eluted, then dialysed Phaseolus vulgaris
1.14.11.2 87.53
-
 purified enzyme, poly-L-proline-eluted Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2
-
 Phaseolus vulgaris poly(4-hydroxyproline) + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 ? x * 65000, SDS-PAGE Phaseolus vulgaris
1.14.11.2 tetramer doublet with MW of 65000, SDS-PAGE Phaseolus vulgaris