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Literature summary extracted from

  • Berg, R.A.; Prockop, D.J.
    Affinity column purification of protocollagen proline hydroxylase from chick embryos and further characterization of the enzyme (1973), J. Biol. Chem., 248, 1175-1182.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 dithiothreitol activation Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 additional information
-
 additional information
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 230000 240000 sedimentation equilibrium centrifugation Gallus gallus
1.14.11.2 350000
-
 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 using affinity chromatography on a column containing a polypeptide substrate of the enzyme linked to agarose, elution of the enzyme with a second peptide substrate and separation of the enzyme from this peptide by gel filtration Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 More the enzyme is dissociated into both monomers and dimers by either dithiothreitol or mercaptoethanol, indicating that the structural integrity of the enzyme is maintained in part by either intrachain or interchain disulfide bonds Gallus gallus
1.14.11.2 tetramer alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
 assay at Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 2-oxoglutarate
-
 Gallus gallus
1.14.11.2 ascorbate
-
 Gallus gallus