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Literature summary extracted from

  • Leushner, J.R.A.; Pasternak, J.
    Partial purification and characterization of prolyl hydroxylase from the free-living nematode Panagrellus silusiae (1978), Can. J. Zool., 56, 159-165.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 catalase activation Panagrellus silusiae
1.14.11.2 dithiothreitol activation Panagrellus silusiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 1,10-phenanthroline
-
 Panagrellus silusiae
1.14.11.2 2,2'-dipyridyl
-
 Panagrellus silusiae
1.14.11.2 poly(L-proline)
-
 Panagrellus silusiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 additional information
-
 additional information
-
 Panagrellus silusiae
1.14.11.2 0.005
-
 Protocollagen
-
 Panagrellus silusiae
1.14.11.2 0.1 0.5 carboxymethylated collagen L-proline
-
 Panagrellus silusiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Panagrellus silusiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 67000
-
 4 * 67000, SDS-PAGE Panagrellus silusiae
1.14.11.2 285000
-
 gel filtration Panagrellus silusiae

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Panagrellus silusiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 partial, using ammonium sulfate precipitation and calcium phosphate gel ion exchange Panagrellus silusiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.2 additional information
-
-
 Panagrellus silusiae

Storage Stability

EC Number Storage Stability Organism
1.14.11.2 -30°C, half-life of ammonium sulfate precipitated enzyme: 7.3 days, stability of enzyme purified with calcium phosphate gel: 21 days, 20-30% loss of activity Panagrellus silusiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 carboxymethylated collagen L-proline + 2-oxoglutarate + O2
-
 Panagrellus silusiae carboxymethylated collagen-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
1.14.11.2 collagen + 2-oxoglutarate + O2
-
 Panagrellus silusiae 4-hydroxyproline containing collagen + succinate + CO2
-
 ?
1.14.11.2 additional information effect of substrate on activity Panagrellus silusiae ?
-
 ?
1.14.11.2 additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Panagrellus silusiae ?
-
 ?
1.14.11.2 protocollagen + 2-oxoglutarate + O2
-
 Panagrellus silusiae 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 tetramer 4 * 67000, SDS-PAGE Panagrellus silusiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
-
 Panagrellus silusiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 7.2
-
-
 Panagrellus silusiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 2-oxoglutarate
-
 Panagrellus silusiae
1.14.11.2 ascorbate
-
 Panagrellus silusiae