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Literature summary extracted from

  • Nietfeld, J.J.; Kemp, A.
    Properties of prolyl 4-hydroxylase containing firmly-bound iron (1980), Biochim. Biophys. Acta, 613, 349-358.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 dithiothreitol activation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 (Pro-Pro-Gly)5 at concentrations higher than 0.56 mM, substrate inhibition observed Gallus gallus
1.14.11.2 EDTA 98-100% inhibition at 0.01 mM Gallus gallus
1.14.11.2 sodium pyrocatechol disulfonate 98-100% inhibition at 0.1 mM Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.008
-
 2-oxoglutarate
-
 Gallus gallus
1.14.11.2 0.4 0.5 (L-Pro-L-Pro-Gly)5
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+ 1 mol/mol, firmly bound, required for catalytic activity, the iron is not part of a 2Fe-2S or a 4Fe-4S cluster Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 58000
-
 2 * 58000 + 2 * 62000, SDS-PAGE Gallus gallus
1.14.11.2 62000
-
 2 * 58000 + 2 * 62000, SDS-PAGE Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.2 additional information
-
 assay method Gallus gallus
1.14.11.2 additional information
-
 enzyme activity after dialysis against iron chelators Gallus gallus
1.14.11.2 0.13
-
 reaction mixture lacking catalase Gallus gallus
1.14.11.2 0.2
-
 reaction mixture lacking serum albumin Gallus gallus
1.14.11.2 0.6
-
 reaction mixture lacking dithiothreitol Gallus gallus
1.14.11.2 0.75
-
 reaction mixture lacking Fe2+ Gallus gallus
1.14.11.2 1.8 2.6
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Gallus gallus (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
1.14.11.2 additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 tetramer 2 * 58000 + 2 * 62000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
 assay at Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.2 additional information
-
 additional information
-
 Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 7.4
-
-
 Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate completely dependent, required for activity Gallus gallus