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Literature summary extracted from

  • Puistola, U.; Turpeenniemi-Hujanen, T.M.; Myllylä, R.; Kivirikko, K.I.
    Studies on the lysyl hydroxylase reaction. I. Initial velocity kinetics and related aspects (1980), Biochim. Biophys. Acta, 611, 40-50.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.4 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine can replace ascorbate Gallus gallus
1.14.11.4 2-mercaptoethanol can replace ascorbate Gallus gallus
1.14.11.4 dithiothreitol can replace ascorbate Gallus gallus
1.14.11.4 L-cysteine can replace ascorbate Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.4 0.04 0.05 O2
-
 Gallus gallus
1.14.11.4 0.05
-
 2-oxoglutarate
-
 Gallus gallus
1.14.11.4 0.09 0.12 2-oxoglutarate
-
 Gallus gallus
1.14.11.4 0.19 0.22 ascorbate
-
 Gallus gallus
1.14.11.4 0.4 0.5 Procollagen L-lysine
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.4 Fe2+ required, Km: 0.001-0.005 mM Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.4 Gallus gallus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.4 embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.4 2-oxoglutarate + O2 + ascorbate uncoupled decarboxylation in absence of peptide substrate Gallus gallus succinate + CO2 + dehydroascorbate + H2O
-
 ?
1.14.11.4 peptidyl-L-lysine + 2-oxoglutarate + O2
-
 Gallus gallus peptidyl-5-hydroxy-L-lysine + succinate + CO2
-
 ?
1.14.11.4 [procollagen] L-lysine + 2-oxoglutarate + O2
-
 Gallus gallus [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.4 ascorbate required Gallus gallus