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Literature summary extracted from

  • McCroskey, R.P.; Griswold, W.R.; Sokoloff, R.L.; Sevier, E.D.; Lin, S.; Liu, C.K.; Shaffer, P.M.; Palmatier, R.D.; Parker, T.S.; Abbott, M.T.
    Studies pertaining to the purification and properties of thymine 7-hydroxylase (1971), Biochim. Biophys. Acta, 277, 264-277.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.6 Fe2+
-
 Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.6 thymine + 2-oxoglutarate + O2 Neurospora crassa
-
 5-hydroxymethyluracil + succinate + CO2
-
 ?
1.14.11.6 thymine + 2-oxoglutarate + O2 Neurospora crassa 1A
-
 5-hydroxymethyluracil + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.6 Neurospora crassa
-
-
-
1.14.11.6 Neurospora crassa 1A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.6
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.6 0.042
-
-
 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.14.11.6 freezing at -79°C, storage at -39°C, 50 mM Tris-HCl buffer, pH 8.0, 1 mM glutathione, 1 mM ascorbate, 10 mM KCl, 8 days, 96% activity Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.6 thymine + 2-oxoglutarate + O2
-
 Neurospora crassa 5-hydroxymethyluracil + succinate + CO2
-
 ?
1.14.11.6 thymine + 2-oxoglutarate + O2
-
 Neurospora crassa 1A 5-hydroxymethyluracil + succinate + CO2
-
 ?