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Literature summary extracted from
- Naphthalene dioxygenase from Pseudomonas NCIB 9816 (1990), Methods Enzymol., 188, 46-52.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.12.12 | 10% ethanol and 10% glycerol are required for stability | Pseudomonas sp. |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.12.12 | cytoplasm | - |
Pseudomonas sp. | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.12 | Fe2+ | - |
Pseudomonas sp. |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.12 | naphthalene + NADH + O2 | Pseudomonas sp. | - |
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.12.12 | Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.12.12 | component B i.e. the terminal oxygenase ISPNAP, DEAE-Sephadex, DEAE-cellulose, octyl-Sepharose, presence of 10% ethanol and 10% glycerol is required to maintain stability | Pseudomonas sp. |
| 1.14.12.12 | ferredoxinNAP reductase, Blue Sepharose, DEAE-cellulose | Pseudomonas sp. |
| 1.14.12.12 | purification of ferredoxinNAP reductase, ferredoxinNAP and ISPNAP | Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.12 | naphthalene + NADH + O2 | - |
Pseudomonas sp. | cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? | |
| 1.14.12.12 | naphthalene + NADH + O2 | - |
Pseudomonas sp. | (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.12 | 7.5 | - |
assay at | Pseudomonas sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.12 | FAD | - |
Pseudomonas sp. | |
| 1.14.12.12 | additional information | spinach ferredoxin cannot replace ferredoxinNAP | Pseudomonas sp. | |
| 1.14.12.12 | NADH | - |
Pseudomonas sp. | |
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