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Literature summary extracted from
- Partial purification and characterization of 5beta-cholestane-3alpha,7alpha,12alpha-triol and 5beta-cholestane-3alpha,7alpha-diol 27-monooxygenase (1982), J. Lipid Res., 23, 345-351.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.15 | 5beta-cholestane-3alpha,7alpha,12alpha-triol | inhibits 5beta-cholestane-3alpha,7alpha-diol 27-monooxygenase activity | Rattus norvegicus |  |
| 1.14.15.15 | 5beta-cholestane-3alpha,7alpha-diol | inhibits 5beta-cholestane-3alpha,7alpha,12alpha-triol 27-monooxygenase activity only slightly | Rattus norvegicus | |
| 1.14.15.15 | Ca2+ | complete inhibition at 1 mM | Rattus norvegicus | |
| 1.14.15.15 | Metyrapone | slight inhibition of 27-monooxygenase | Rattus norvegicus | |
| 1.14.15.15 | p-chloromercuribenzoate | complete inhibition at 1 mM | Rattus norvegicus | |
| 1.14.15.15 | Phenyl isocyanide | complete inhibition at 1 mM | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.15.15 | 0.0045 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus | |
| 1.14.15.15 | 0.01 | - |
5beta-cholestane-3alpha,7alpha-diol | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.15 | mitochondrial membrane | - |
Rattus norvegicus | 31966 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.15 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.15 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.15 | liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.15 | 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | 5beta-cholestane-3alpha,7alpha,12alpha,27-tetrol + oxidized adrenodoxin + H2O | - |
? | |
| 1.14.15.15 | 5beta-cholestane-3alpha,7alpha-diol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | ? | - |
? | |
| 1.14.15.15 | 5beta-cholestane-3alpha-ol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.15 | 5beta-cholestane-3alpha,7alpha,12alpha-triol 27-monooxygenase | enzyme was renamed into 27-monooxygenase because it hydroxylates the methyl group in position 27 | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.15 | 7.7 | - |
- |
Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.15 | 6.5 | 8 | 27-hydroxylation | Rattus norvegicus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.15.15 | 0.004 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus | |
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