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Literature summary extracted from

  • Wang, H.; Tiirola, M.A.; Puhakka, J.A.; Kulomaa, M.S.
    Production and characterization of the recombinant Sphingomonas chlorophenolica pentachlorophenol 4-monooxygenase (2001), Biochem. Biophys. Res. Commun., 289, 161-166.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.50 pcpB gene, expression of wild-type and mutant in Escherichia coli as glutathione-S-transferase fusion protein Flavobacterium sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.50 additional information introduction of tobacco etch virus protease cleavage site into the enzyme as a glutathione-S-transferase fusion protein Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.50 0.05
-
 Pentachlorophenol recombinant enzyme Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.50 Flavobacterium sp.
-
 identical with Sphingomonas chlorophenolica strain ATCC 39723
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.50 recombinant wild-type and mutant from Escherichia coli as glutathione-S-transferase fusion proteins Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.50 pentachlorophenol + NADPH + H+ + O2
-
 Flavobacterium sp. 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.50 PCP4MO
-
 Flavobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.50 FAD recombinant enzyme has no FAD, activity only in presence of added FAD Flavobacterium sp.