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Literature summary extracted from

  • Abita, J.P.; Parniak, M.; Kaufman, S.
    The activation of rat liver phenylalanine hydroxylase by limited proteolysis, lysolecithin, and tocopherol phosphate. Changes in conformation and catalytic properties (1984), J. Biol. Chem., 259, 14560-14566.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.1 alpha-chymotrypsin limited proteolysis of purified liver enzyme, 20-30fold increase in activity, cofactor tetrahydrobiopterin Rattus norvegicus
1.14.16.1 liver lysosomal proteases limited proteolysis of liver enzyme Rattus norvegicus
1.14.16.1 lysolecithin
-
 Rattus norvegicus
1.14.16.1 additional information relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis Rattus norvegicus
1.14.16.1 Phospholipids activate Rattus norvegicus
1.14.16.1 Phospholipids increase in activity in the presence of tetrahydrobiopterin but not in the presence of synthetic pterin cofactors Rattus norvegicus
1.14.16.1 Trypsin limited proteolysis of purified liver enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.1 0.002
-
 tetrahydrobiopterin native liver enzyme Rattus norvegicus
1.14.16.1 0.012
-
 tetrahydrobiopterin chymotrypsin activated liver enzyme Rattus norvegicus
1.14.16.1 0.045
-
 6-methyltetrahydropterin native liver enzyme Rattus norvegicus
1.14.16.1 0.065
-
 6,7-dimethyltetrahydrobiopterin native liver enzyme Rattus norvegicus
1.14.16.1 0.065
-
 6,7-dimethyltetrahydrobiopterin native enzyme, at pH 6.8 and 25°C Rattus norvegicus
1.14.16.1 0.087
-
 6-methyltetrahydropterin chymotrypsin activated liver enzyme Rattus norvegicus
1.14.16.1 0.105
-
 6,7-dimethyltetrahydrobiopterin chymotrypsin activated liver enzyme Rattus norvegicus
1.14.16.1 0.105
-
 6,7-dimethyltetrahydrobiopterin chymotrypsin-activated enzyme, at pH 6.8 and 25°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.1 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 L-phenylalanine + 6,7-dimethyltetrahydrobiopterin
-
 Rattus norvegicus L-tyrosine + 6,7-dimethyl-4a-hydroxy-tetrahydrobiopterin
-
 r
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.1 tetrahydrobiopterin
-
 Rattus norvegicus