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Literature summary extracted from

  • Nakata, H.; Fujisawa, H.
    Purification and characterization of phenylalanine 4-monooxygenase from rat liver (1980), Biochim. Biophys. Acta, 614, 313-327.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.1 0.0344
-
 6,7-dimethyl-5,6,7,8-tetrahydropterin enzyme form II, substrate L-phenylalanine Rattus norvegicus
1.14.16.1 0.037
-
 6-Methyl-5,6,7,8-tetrahydropterin enzyme form I, substrate L-phenylalanine Rattus norvegicus
1.14.16.1 0.0444
-
 6,7-dimethyl-5,6,7,8-tetrahydropterin enzyme form I, substrate L-phenylalanine Rattus norvegicus
1.14.16.1 0.0455
-
 6-Methyl-5,6,7,8-tetrahydropterin enzyme form II, substrate L-phenylalanine Rattus norvegicus
1.14.16.1 0.454
-
 L-phenylalanine cofactor 6-methyl-5,6,7,8-tetrahydropterin, enzyme form II Rattus norvegicus
1.14.16.1 0.72
-
 L-phenylalanine cofactor 6-methyl-5,6,7,8-tetrahydropterin, enzyme form I Rattus norvegicus
1.14.16.1 1.43
-
 L-phenylalanine cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin, enzyme form II Rattus norvegicus
1.14.16.1 1.92
-
 L-phenylalanine cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin, enzyme form I Rattus norvegicus
1.14.16.1 4.9
-
 L-tryptophan cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Rattus norvegicus
1.14.16.1 8.5
-
 L-tryptophan cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, enzyme form I Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.1 Iron iron-protein Rattus norvegicus
1.14.16.1 Iron 0.6 mol of iron per mol of 51000 Da subunit Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.1 51000
-
 4 * 51000, enzyme form I and II, SDS-PAGE Rattus norvegicus
1.14.16.1 240000
-
 sedimentation equilibrium Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.16.1 side-chain modification enzyme form I contains 0.3 mol phosphate per mol subunit, enzyme form II contains 0.4 mol Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.1 distinct forms I and II differing in phosphate content and isoelectric point Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.1 0.59
-
-
 Rattus norvegicus
1.14.16.1 1.46
-
-
 Rattus norvegicus
1.14.16.1 1.46
-
 enzyme form II Rattus norvegicus
1.14.16.1 1.8
-
 enzyme form I Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.14.16.1 -80°C, 1 month, 30% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?
1.14.16.1 L-tryptophan + tetrahydrobiopterin + O2
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.1 tetramer 4 * 51000, enzyme form I and II, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.16.1 9.08
-
 phenylalanine cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.1 tetrahydrobiopterin
-
 Rattus norvegicus