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Literature summary extracted from

  • Daubner, S.C.; Fitzpatrick, P.F.
    Mutation to phenylalanine of tyrosine 371 in tyrosine hydroxylase increases the affinity for phenylalanine (1998), Biochemistry, 37, 16440-16444.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.2 expression of wild-type and mutant enzymes in Escherichia coli Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.2 Y371F increased Km for tyrosine and pterin cosubstrates, highly decreased Km for phenylalanine Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.2 0.01
-
 phenylalanine recombinant mutant Y371F Rattus norvegicus
1.14.16.2 0.027
-
 Tetrahydropterin recombinant wild-type, pH 7.1 Rattus norvegicus
1.14.16.2 0.032
-
 tyrosine recombinant wild-type, pH 6.0 Rattus norvegicus
1.14.16.2 0.033
-
 6-Methyl-5,6,7,8-tetrahydropterin recombinant wild-type, pH 7.1 Rattus norvegicus
1.14.16.2 0.051
-
 tyrosine recombinant wild-type, pH 7.1 Rattus norvegicus
1.14.16.2 0.053
-
 Tetrahydropterin recombinant mutant Y371F, pH 7.1 Rattus norvegicus
1.14.16.2 0.055
-
 6-Methyl-5,6,7,8-tetrahydropterin recombinant wild-type, pH 6.0 Rattus norvegicus
1.14.16.2 0.059
-
 6-Methyl-5,6,7,8-tetrahydropterin recombinant mutant Y371F, pH 7.1 Rattus norvegicus
1.14.16.2 0.065
-
 tyrosine recombinant mutant Y371F, pH 7.1 Rattus norvegicus
1.14.16.2 0.065
-
 6-Methyl-5,6,7,8-tetrahydropterin recombinant mutant Y371F, pH 6.0 Rattus norvegicus
1.14.16.2 0.071
-
 tyrosine recombinant mutant Y371F, pH 6.0 Rattus norvegicus
1.14.16.2 0.11
-
 phenylalanine recombinant wild-type Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.2 Iron
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Rattus norvegicus P04177
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.16.2 L-tyrosine + a 5,6,7,8-tetrahydropteridine + O2 = L-dopa + a 4a-hydroxy-5,6,7,8-tetrahydropteridine mechanism Rattus norvegicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-phenylalanine + tetrahydropteridine + O2 recombinant wild-type and mutant Rattus norvegicus L-tyrosine + dihydropteridine + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 recombinant wild-type and mutant Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 TH
-
 Rattus norvegicus