EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.16.2 | 4 isoforms, overexpression in Escherichia coli, phosphorylation-free | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.2 | tyrosine | substrate inhibition, with (6R)-L-erythro-tetrahydrobiopterin | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.16.2 | additional information | - |
additional information | Km dependent on cosubstrate type and concentration, overview for 4 isoforms | Homo sapiens | |
1.14.16.2 | 0.055 | - |
tyrosine | recombinant isoform hTH2, with 6-methyltetrahydropterin | Homo sapiens | |
1.14.16.2 | 0.066 | - |
tyrosine | recombinant isoform hTH4, with 6-methyltetrahydropterin | Homo sapiens | |
1.14.16.2 | 0.074 | - |
tyrosine | recombinant isoform hTH3, with 6-methyltetrahydropterin | Homo sapiens | |
1.14.16.2 | 0.166 | - |
tyrosine | recombinant isoform hTH1, with 6-methyltetrahydropterin | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.2 | Iron | required | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 24000 | - |
4 isoforms, gel filtration | Homo sapiens |
1.14.16.2 | 60000 | - |
4 * 60000, 4 isoforms, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.2 | L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 | Homo sapiens | probable regulatory role of cosubstrate for all 4 isoforms | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.16.2 | Homo sapiens | - |
4 isoforms hTH1, hTH2, hTH3, hTH4 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.16.2 | 4 isoforms, recombinant from Escherichia coli | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.2 | L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 | cosubstrate has a regulatory role for all 4 isoforms | Homo sapiens | ? | - |
? | |
1.14.16.2 | L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 | probable regulatory role of cosubstrate for all 4 isoforms | Homo sapiens | ? | - |
? | |
1.14.16.2 | L-tyrosine + (6RS)-L-erythro-tetrahydrobiopterin + O2 | - |
Homo sapiens | ? | - |
? | |
1.14.16.2 | L-tyrosine + (6S)-L-erythro-tetrahydrobiopterin + O2 | - |
Homo sapiens | ? | - |
? | |
1.14.16.2 | L-tyrosine + 6-methyltetrahydropterin + O2 | - |
Homo sapiens | ? | - |
? | |
1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | - |
Homo sapiens | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.16.2 | tetramer | 4 * 60000, 4 isoforms, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.16.2 | TH | - |
Homo sapiens |
1.14.16.2 | tyrosine-3-mono-oxygenase | - |
Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 6 | 7 | 4 isoforms | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 5 | 8.5 | 4 isoforms | Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 7 | - |
preincubation at 37°C for 2 h below, enzyme isoforms are unstable, especially isoforms hTH1 and hTH3 | Homo sapiens |
1.14.16.2 | 7 | - |
preincubation at 37°C for 2 h above, isoforms are stable | Homo sapiens |
1.14.16.2 | 8 | - |
isoforms, optimal stability at | Homo sapiens |