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Literature summary extracted from

  • Tong, J.H.; Kaufman, S.
    Tryptophan hydroxylase. Purification and some properties of the enzyme from rabbit hindbrain (1975), J. Biol. Chem., 250, 4152-4158.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.4 L-phenylalanine substrate inhibition with tetrahydrobiopterin as cofactor, above 0.5 mM Oryctolagus cuniculus
1.14.16.4 L-tryptophan substrate inhibition with 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine, not with 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine, above 0.2 mM Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.4 0.032
-
 L-tryptophan cofactor tetrahydrobiopterin Oryctolagus cuniculus
1.14.16.4 0.286
-
 phenylalanine cofactor tetrahydrobiopterin Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.4 57700
-
 2 * 57700 + 2 * 60900, hindbrain enzyme, SDS-PAGE Oryctolagus cuniculus
1.14.16.4 60900
-
 2 * 57700 + 2 * 60900, hindbrain enzyme, SDS-PAGE Oryctolagus cuniculus
1.14.16.4 230000
-
 gel filtration, PAGE, sucrose density gradient centrifugation Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Oryctolagus cuniculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.16.4 additional information less than 1% bound carbohydrate Oryctolagus cuniculus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4 hindbrain enzyme, calcium phosphate gel, polyethylene glycol 6000, Sepharose 6B, DEAE-cellulose Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.4 brain hindbrain Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.4 0.0021
-
 hindbrain enzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-phenylalanine + tetrahydropteridine + O2 28.8% of the rate observed with L-tryptophan Oryctolagus cuniculus tyrosine + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + tetrahydropteridine + O2 cofactor tetrahydrobiopterin Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + tetrahydropteridine + O2 cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.4 tetramer 2 * 57700 + 2 * 60900, hindbrain enzyme, SDS-PAGE Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.16.4 0.197
-
 L-phenylalanine
-
 Oryctolagus cuniculus