Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hosoda, S.; Nakamura, W.; Takatsuki, K.
    Properties of tryptophan hydroxylase from human carcinoid tumor (1977), Biochim. Biophys. Acta, 482, 27-34.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.4 1,10-phenanthroline 0.0024 mM, 50% inhibition, competitive vs. tryptophan Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.4 additional information
-
 additional information 1.2% O2 with 0.2 mM 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine, 7.1% O2 with 0.4 mM 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine Homo sapiens
1.14.16.4 0.05
-
 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine
-
 Homo sapiens
1.14.16.4 0.105
-
 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.4 Fe2+ not required for full activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Homo sapiens
-
 enzyme from a carcinoid tumor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.4 carcinoma cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.4 0.000018
-
 enzyme from carconoid tumor Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.14.16.4 4°C, under N2, 2 months, no loss of activity Homo sapiens
1.14.16.4 4°C, under O2, 48 h, 90% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-tryptophan + 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine + O2
-
 Homo sapiens ?
-
 r
1.14.16.4 L-tryptophan + tetrahydropteridine + O2
-
 Homo sapiens 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.4 6.4
-
 in the presence of 0.2 mM Fe2+ and 2-mercaptoethanol Homo sapiens
1.14.16.4 6.8
-
 in the presence of 0.2 mM Fe2+, without 2-mercaptoethanol Homo sapiens
1.14.16.4 7.2
-
 in the presence of 35.5 mM 2-mercaptoethanol Homo sapiens
1.14.16.4 7.6
-
 without 2-mercaptoethanol and Fe2+ Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.16.4 0.00082
-
 1,10-phenanthroline vs. tryptophan Homo sapiens
1.14.16.4 0.0026
-
 1,10-phenanthroline vs. 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine Homo sapiens