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Literature summary extracted from
- Purification and properties of tryptophan 5-monooxygenase from rat brain-stem (1982), Eur. J. Biochem., 122, 41-47.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.4 | 0.119 | - |
L-tryptophan | - |
Rattus norvegicus |  |
| 1.14.16.4 | 0.125 | - |
2-amino-4-hydroxy-6-methyltetrahydropteridine | - |
Rattus norvegicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.4 | Fe2+ | 0.02 mM, 3.5fold increase in activity | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 59000 | - |
4 * 59000, tryptophan hydroxylase form I from brain stem, SDS-PAGE | Rattus norvegicus |
| 1.14.16.4 | 288000 | - |
- |
Rattus norvegicus |
| 1.14.16.4 | 300000 | - |
- |
Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.4 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.4 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.16.4 | brain | stem | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 0.082 | - |
tryptophan hydroxylase form II | Rattus norvegicus |
| 1.14.16.4 | 0.374 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.4 | L-phenylalanine + tetrahydropteridine + O2 | tryptophan hydroxylase form I, 39% of activity with L-tryptophan | Rattus norvegicus | tyrosine + dihydropteridine + H2O | - |
? | |
| 1.14.16.4 | L-tryptophan + 2-amino-4-hydroxy-6-methyl-tetrahydropteridine + O2 | - |
Rattus norvegicus | ? | - |
r | |
| 1.14.16.4 | L-tryptophan + tetrahydropteridine + O2 | - |
Rattus norvegicus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.16.4 | tetramer | 4 * 59000, tryptophan hydroxylase form I from brain stem, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 7.6 | - |
- |
Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.4 | 6 | 9.5 | about 50% activity at pH 6 and 9.5 | Rattus norvegicus |
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