EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.4 | dithiothreitol | or other sulfhydryl reagents like 2-mercaptoethanol and L-cysteine are essential for activation, 5fold increase in activity | Mus musculus |
EC Number | General Stability | Organism |
---|---|---|
1.14.16.4 | EDTA stabilizes | Mus musculus |
1.14.16.4 | ethylene glycol, 50%, stabilizes | Mus musculus |
1.14.16.4 | NaCl: 1 M, in addition to other stabilizing agents brings a great improvement in stabilization | Mus musculus |
1.14.16.4 | Tween 20 stabilizes | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.4 | 1,10-phenanthroline | - |
Mus musculus | |
1.14.16.4 | 2,2'-dipyridyl | - |
Mus musculus | |
1.14.16.4 | 3,4-dihydroxyphenylalanine | - |
Mus musculus | |
1.14.16.4 | 3,4-Dihydroxyphenylethylamine | - |
Mus musculus | |
1.14.16.4 | 4-Fluorotryptophan | - |
Mus musculus | |
1.14.16.4 | 5-Fluorotryptophan | - |
Mus musculus | |
1.14.16.4 | 5-hydroxytryptophan | - |
Mus musculus | |
1.14.16.4 | 6-Fluorotryptophan | - |
Mus musculus | |
1.14.16.4 | 8-hydroxyquinoline | - |
Mus musculus | |
1.14.16.4 | L-4-chlorophenylalanine | - |
Mus musculus | |
1.14.16.4 | L-p-Chlorophenylalanine | - |
Mus musculus | |
1.14.16.4 | L-phenylalanine | competitive vs. tryptophan | Mus musculus | |
1.14.16.4 | L-tryptophan | competitive to L-phenylalanine | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.16.4 | 0.045 | - |
L-tryptophan | cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine | Mus musculus | |
1.14.16.4 | 0.055 | - |
2-amino-4-hydroxy-6-methyltetrahydropteridine | substrate L-tryptophan | Mus musculus | |
1.14.16.4 | 0.1 | - |
L-phenylalanine | cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine | Mus musculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.4 | Fe2+ | 5fold increase in activity | Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.16.4 | 53000 | - |
4 * 53000, enzyme from mastocytoma cells, SDS-PAGE | Mus musculus |
1.14.16.4 | 270000 | - |
enzyme from mastocytoma cells, gel filtration | Mus musculus |
1.14.16.4 | 280000 | - |
enzyme from mastocytoma cells, gradient PAGE | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.16.4 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.16.4 | - |
Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.16.4 | mastocytoma cell | - |
Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.16.4 | 5.28 | - |
purified from mastocytoma cells | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.4 | L-phenylalanine + tetrahydropteridine + O2 | - |
Mus musculus | tyrosine + dihydropteridine + H2O | - |
? | |
1.14.16.4 | L-tryptophan + 2-amino-4-hydroxy-6-methyl-tetrahydropteridine + O2 | - |
Mus musculus | ? | - |
r | |
1.14.16.4 | L-tryptophan + tetrahydropteridine + O2 | - |
Mus musculus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.16.4 | tetramer | 4 * 53000, enzyme from mastocytoma cells, SDS-PAGE | Mus musculus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.16.4 | 4 | - |
complete loss of activity in absence of stabilizing agents after 20 h | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.16.4 | 7.2 | - |
- |
Mus musculus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.16.4 | 6 | 8.7 | about 50% activity at pH 6.0 and 8.7 | Mus musculus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.16.4 | 0.026 | - |
L-4-chlorophenylalanine | - |
Mus musculus |