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Literature summary extracted from

  • Nukiwa, T.; Tohyama, C.; Okita, C.; Kataoka, T.; Ichiyama, A.
    Purification and some properties of bovine pineal tryptophan 5-monooxygenase (1974), Biochem. Biophys. Res. Commun., 60, 1029-1035.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.4 6-Fluorotryptophan 1 mM, 90% inhibition of pineal tryptophan hydroxylase Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.4 0.016
-
 L-tryptophan enzyme from pineal gland, cofactor tetrahydrobiopterin Bos taurus
1.14.16.4 0.032
-
 L-phenylalanine enzyme from pineal gland, cofactor tetrahydrobiopterin Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.4 30000
-
 enzyme from pineal gland, gel filtration, sucrose density gradient ultracentrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4 enzyme from pineal gland, ammonium sulfate, hydroxylapatite, P-cellulose, DEAE-cellulose, Sephadex G-200 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.16.4 L-tryptophan + a 5,6,7,8-tetrahydropteridine + O2 = 5-hydroxy-L-tryptophan + a 4a-hydroxy-5,6,7,8-tetrahydropteridine phenylalanine hydroxylase, EC 1.14.16.1 also catalyzes this reaction Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.4 pineal gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.4 0.235
-
 tryptophan hydroxylase from pineal gland Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-phenylalanine + tetrahydropteridine + O2 at a comparable rate of L-tryptophan hydroxylation Bos taurus tyrosine + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + tetrahydropteridine + O2
-
 Bos taurus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?