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Literature summary extracted from

  • Nagai, J.; Bloch, K.
    Enzymatic desaturation of stearyl acyl carrier protein (1968), J. Biol. Chem., 243, 4626-4633.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.2 FAD 0.005 mM, 74% inhibition Euglena gracilis
1.14.19.2 FMN 0.006 mM, 73% inhibition Euglena gracilis
1.14.19.2 KCN 0.01 mM, 50% inhibition, 0.1 mM, 80% inhibition Euglena gracilis
1.14.19.2 additional information not inhibited by CO Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.2 soluble
-
 Euglena gracilis
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.2 Euglena gracilis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.2 stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O enzyme acts on derivatives of a number of long-chain fatty acids, requires ferredoxin, formerly EC 1.14.99.6 Euglena gracilis
a

Storage Stability

EC Number Storage Stability Organism
1.14.19.2 0°C, 10 h, 50% loss of activity Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.2 stearoyl-CoA + electron donor + O2 purified reconstituted enzyme shows same activity as with stearoyl-[acyl-carrier-protein], no activity in crude extracts Euglena gracilis 9-octadecenoyl-CoA + acceptor + H2O
-
 ?
1.14.19.2 stearoyl-[acyl-carrier protein] + reduced acceptor + O2 no activity with NADH Euglena gracilis oleoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?
1.14.19.2 stearoyl-[acyl-carrier protein] + reduced acceptor + O2 electron donor NADPH Euglena gracilis oleoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?
1.14.19.2 stearoyl-[acyl-carrier protein] + reduced acceptor + O2 purified enzyme is not active with NADH Euglena gracilis oleoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?
1.14.19.2 stearoyl-[acyl-carrier protein] + reduced acceptor + O2 enzyme acts on derivatives of a number of long chain fatty acids Euglena gracilis oleoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.2 Ferredoxin stearyl acyl carrier protein desaturase system consists of 3 components: a reduced NADPH-oxidoreductase, a desaturase and ferredoxin Euglena gracilis
1.14.19.2 additional information NADH is ineffective as electron donor Euglena gracilis
1.14.19.2 NADPH
-
 Euglena gracilis