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Literature summary extracted from
- Purification of prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes (1976), J. Biol. Chem., 251, 2629-2636.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.1 | benzoquinone | stimulates conversion of prostaglandin G1 to H1 | Bos taurus |  |
| 1.14.99.1 | epinephrine | stimulates conversion of prostaglandin G1 to H1 | Bos taurus | |
| 1.14.99.1 | hydroquinone | stimulates conversion of prostaglandin G1 to H1 | Bos taurus | |
| 1.14.99.1 | indole | stimulates conversion of prostaglandin G1 to H1 | Bos taurus | |
| 1.14.99.1 | kynurenine | stimulates conversion of prostaglandin G1 to H1 | Bos taurus | |
| 1.14.99.1 | Melatonin | stimulates | Bos taurus | |
| 1.14.99.1 | phenylalanine | stimulates conversion of prostaglandin G1 to H1 | Bos taurus | |
| 1.14.99.1 | serotonin | stimulates conversion of prostaglandin G1 to H1 | Bos taurus | |
| 1.14.99.1 | tryptophan | stimulates conversion of prostaglandin G1 to H1 | Bos taurus | |
| 1.14.99.1 | tyrosine | stimulates conversion of prostaglandin G1 to H1 | Bos taurus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.1 | Acetylsalicylic acid | inhibition of prostaglandin G1 synthesis | Bos taurus | |
| 1.14.99.1 | indomethacin | inhibition of prostaglandin G1 synthesis | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.99.1 | microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 300000 | 350000 | gel filtration | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.1 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.99.1 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.99.1 | seminal vesicle | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 2.4 | - |
prostaglandin H1 synthesis | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.1 | 8,11,14-eicosatrienoic acid + O2 | bis-dioxygenase activity, cyclooxygenase activity, presence of hematin | Bos taurus | prostaglandin G1 + ? | 9alpha,11alpha-epidioxy-15(S)-hydroperoxy-13-trans-prostenoic acid | ? | |
| 1.14.99.1 | 8,11,14-eicosatrienoic acid + O2 | hydroperoxidase activity, presence of hematin and tryptophan | Bos taurus | prostaglandin H1 + ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 50 | - |
pH 8.0, 5 min, complete loss of activity | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 8 | - |
synthesis of prostaglandin G1, conversion to prostaglandin H1 | Bos taurus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 6 | 8 | 24°C, 5 min, stable | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.1 | heme | either free or protein-bound heme is required for cyclooxygenase- and hydroperoxidase activity | Bos taurus | |
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Release 2023.1 (January 2023)
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