EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.99.20 | wild-type and mutants of bovine enzyme species expressed in baculovirus-infected Spodoptera frugiperda cells | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.99.20 | microsome | solubilized microsomal recombinant protein preparation from baculovirus-infected Spodoptera frugiperda cells | Bos taurus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.99.20 | 94000 | - |
x * 94000, SDS-PAGE, wild type enzyme | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.20 | phylloquinone + electron donor + O2 | Bos taurus | - |
2,3-epoxyphylloquinone + oxidized electron + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.20 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.99.20 | partially | Bos taurus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.99.20 | phylloquinone + reduced acceptor + O2 = 2,3-epoxyphylloquinone + acceptor + H2O | enzyme has two distinct functions: vitamin K epoxidation and gamma-glutamyl carboxylation | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.99.20 | liver | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.99.20 | additional information | - |
- |
Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.99.20 | -80°C, soluble enzyme preparation, at least 5% glycerol, stable to multiple freeze and thaw cycles | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.20 | phylloquinone + electron donor + O2 | - |
Bos taurus | 2,3-epoxyphylloquinone + oxidized electron donor + H2O | - |
? | |
1.14.99.20 | phylloquinone + electron donor + O2 | - |
Bos taurus | 2,3-epoxyphylloquinone + oxidized electron + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.99.20 | ? | x * 94000, SDS-PAGE, wild type enzyme | Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.99.20 | 25 | - |
enzyme assay | Bos taurus |