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Literature summary extracted from

  • Fujii, J.; Myint, T.; Seo, H.G.; Kyanoki, Y.; Ikeda, Y.; Taniguchi, N.
    Characterization of wild-type and amyotrophic lateral sclerosis-related mutant Cu,Zn-superoxide dismutases overproduced in baculovirus-infected insect cells (1995), J. Neurochem., 64, 1456-1461.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.15.1.1 medicine investigations with help of mutants to elucidate structure-function relation in familial amyotrophic lateral sclerosis Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 Cu,Zn-SOD, overexpression of wild-type and mutants in Spodoptera frugiperda cells Sf21 via baculovirus infection Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.15.1.1 G41N Cu,Zn-SOD, site-directed mutagenesis, analogous to mutant found in familial amyotrophic lateral sclerosis, 47% activity compared to the wild-type Homo sapiens
1.15.1.1 G85R Cu,Zn-SOD, site-directed mutagenesis, analogous to mutant found in familial amyotrophic lateral sclerosis, 99% activity compared to the wild-type Homo sapiens
1.15.1.1 H43R Cu,Zn-SOD, site-directed mutagenesis, analogous to mutant found in familial amyotrophic lateral sclerosis, 66% activity compared to the wild-type Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+
-
 Homo sapiens
1.15.1.1 Zn2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Homo sapiens
-
 Cu,Zn-SOD
-
1.15.1.1 Homo sapiens CuZn-SOD
-
 Cu,Zn-SOD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 Cu,Zn-SOD, wild-type and mutants recombinant from Spodoptera frugiperda cells Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.15.1.1 apoprotein expressed in insect cells can be restored by addition of Cu2+, fully active Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 erythrocyte Cu,Zn-SOD Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2- + H+
-
 Homo sapiens O2 + H2O2
-
 ?
1.15.1.1 O2- + H+
-
 Homo sapiens CuZn-SOD O2 + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 37
-
 wild-type, purified stable for at least 1 week Homo sapiens